Chromosomal restriction fragment length polymorphism analysis of Escherichia coli strains causing recurrent urinary tract infections in young women

T. A. Russo, A. Stapleton, S. Wenderoth, Thomas Hooton, W. E. Stamm

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

To determine whether recurrent, symptomatic urinary tract infections (UTIs) in a given individual are due to the same or different strains, 71 Escherichia coli strains that caused recurrent UTIs were prospectively collected from 23 infection-prone young women and studied by chromosomal restriction fragment length polymorphism (RFLP) analysis using pulsed-field gel electrophoresis. Thirty-five strains from women with first-episode UTIs were also studied. Overall, 30 (68%) of 44 recurrent UTIs were caused by a strain previously identified in that person. In contrast, 32 of 35 strains from first-episode UTIs had unique RFLP profiles. Analysis of a subset of subjects established that the majority of recurrent UTIs were due to reinfection, not persistence of the pathogen within the urinary tract, and suggested that the colonic flora was the reservoir for these reinfecting strains.

Original languageEnglish
Pages (from-to)440-445
Number of pages6
JournalJournal of Infectious Diseases
Volume172
Issue number2
StatePublished - Jan 1 1995
Externally publishedYes

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Urinary Tract Infections
Restriction Fragment Length Polymorphisms
Escherichia coli
Pulsed Field Gel Electrophoresis
Urinary Tract
Infection

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Chromosomal restriction fragment length polymorphism analysis of Escherichia coli strains causing recurrent urinary tract infections in young women. / Russo, T. A.; Stapleton, A.; Wenderoth, S.; Hooton, Thomas; Stamm, W. E.

In: Journal of Infectious Diseases, Vol. 172, No. 2, 01.01.1995, p. 440-445.

Research output: Contribution to journalArticle

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