Abstract
Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is an important technique in the study of protein-gene interactions. Using CMP, DNA-protein interactions are studied within the context of the cell The basic steps in this technique are fixation, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. Although ChIP is a very versatile tool, the procedure requires the optimization of reaction conditions. Several modifications to the original ChIP technique have been published to improve the success and to enhance the utility of this procedure. This review addresses the critical parameters and the variants of ChIP as well as the different analytical tools that can be combined with ChIP to enable better understanding of DNA-protein interactions in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 961-969 |
| Number of pages | 9 |
| Journal | BioTechniques |
| Volume | 37 |
| Issue number | 6 |
| State | Published - Dec 1 2004 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Chromatin immunoprecipitation assay. / Das, Partha M.; Ramachandran, Kavitha; VanWert, Jane; Singal, Rakesh.
In: BioTechniques, Vol. 37, No. 6, 01.12.2004, p. 961-969.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chromatin immunoprecipitation assay
AU - Das, Partha M.
AU - Ramachandran, Kavitha
AU - VanWert, Jane
AU - Singal, Rakesh
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is an important technique in the study of protein-gene interactions. Using CMP, DNA-protein interactions are studied within the context of the cell The basic steps in this technique are fixation, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. Although ChIP is a very versatile tool, the procedure requires the optimization of reaction conditions. Several modifications to the original ChIP technique have been published to improve the success and to enhance the utility of this procedure. This review addresses the critical parameters and the variants of ChIP as well as the different analytical tools that can be combined with ChIP to enable better understanding of DNA-protein interactions in vivo.
AB - Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is an important technique in the study of protein-gene interactions. Using CMP, DNA-protein interactions are studied within the context of the cell The basic steps in this technique are fixation, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. Although ChIP is a very versatile tool, the procedure requires the optimization of reaction conditions. Several modifications to the original ChIP technique have been published to improve the success and to enhance the utility of this procedure. This review addresses the critical parameters and the variants of ChIP as well as the different analytical tools that can be combined with ChIP to enable better understanding of DNA-protein interactions in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=10044219622&partnerID=8YFLogxK
M3 - Article
C2 - 15597545
AN - SCOPUS:10044219622
VL - 37
SP - 961
EP - 969
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 6
ER -