Chromatin immunoprecipitation assay

Partha M. Das, Kavitha Ramachandran, Jane VanWert, Rakesh Singal

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is an important technique in the study of protein-gene interactions. Using CMP, DNA-protein interactions are studied within the context of the cell The basic steps in this technique are fixation, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. Although ChIP is a very versatile tool, the procedure requires the optimization of reaction conditions. Several modifications to the original ChIP technique have been published to improve the success and to enhance the utility of this procedure. This review addresses the critical parameters and the variants of ChIP as well as the different analytical tools that can be combined with ChIP to enable better understanding of DNA-protein interactions in vivo.

Original languageEnglish
Pages (from-to)961-969
Number of pages9
JournalBioTechniques
Volume37
Issue number6
StatePublished - Dec 1 2004

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Chromatin Immunoprecipitation
Chromatin
Assays
DNA
Proteins
Cytidine Monophosphate
Recombinational DNA Repair
Chromosomal Instability
Sonication
DNA-Binding Proteins
Transcription
DNA Replication
Immunoprecipitation
Epigenomics
Gene expression
Genes
Cell Cycle
Repair
Cells
Cell Proliferation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Das, P. M., Ramachandran, K., VanWert, J., & Singal, R. (2004). Chromatin immunoprecipitation assay. BioTechniques, 37(6), 961-969.

Chromatin immunoprecipitation assay. / Das, Partha M.; Ramachandran, Kavitha; VanWert, Jane; Singal, Rakesh.

In: BioTechniques, Vol. 37, No. 6, 01.12.2004, p. 961-969.

Research output: Contribution to journalArticle

Das, PM, Ramachandran, K, VanWert, J & Singal, R 2004, 'Chromatin immunoprecipitation assay', BioTechniques, vol. 37, no. 6, pp. 961-969.
Das PM, Ramachandran K, VanWert J, Singal R. Chromatin immunoprecipitation assay. BioTechniques. 2004 Dec 1;37(6):961-969.
Das, Partha M. ; Ramachandran, Kavitha ; VanWert, Jane ; Singal, Rakesh. / Chromatin immunoprecipitation assay. In: BioTechniques. 2004 ; Vol. 37, No. 6. pp. 961-969.
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