Abstract
This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.
Original language | English (US) |
---|---|
Pages (from-to) | 226-230 |
Number of pages | 5 |
Journal | Brain research |
Volume | 903 |
Issue number | 1-2 |
DOIs | |
State | Published - Jun 8 2001 |
Externally published | Yes |
Keywords
- Cholinergic
- Heat shock
- Oxidative stress
- Presenilin
- Signaling
ASJC Scopus subject areas
- Neuroscience(all)