Abstract
This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.
| Original language | English |
|---|---|
| Pages (from-to) | 226-230 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 903 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jun 8 2001 |
| Externally published | Yes |
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Keywords
- Cholinergic
- Heat shock
- Oxidative stress
- Presenilin
- Signaling
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1. / De Sarno, Patrizia; Lesort, Mathieu; Bijur, Gautam N.; Johnson, Gail V W; Jope, Richard S.
In: Brain Research, Vol. 903, No. 1-2, 08.06.2001, p. 226-230.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1
AU - De Sarno, Patrizia
AU - Lesort, Mathieu
AU - Bijur, Gautam N.
AU - Johnson, Gail V W
AU - Jope, Richard S
PY - 2001/6/8
Y1 - 2001/6/8
N2 - This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.
AB - This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.
KW - Cholinergic
KW - Heat shock
KW - Oxidative stress
KW - Presenilin
KW - Signaling
UR - http://www.scopus.com/inward/record.url?scp=0035827090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035827090&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(01)02428-3
DO - 10.1016/S0006-8993(01)02428-3
M3 - Article
C2 - 11382407
AN - SCOPUS:0035827090
VL - 903
SP - 226
EP - 230
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -