Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1

Patrizia De Sarno, Mathieu Lesort, Gautam N. Bijur, Gail V.W. Johnson, Richard S. Jope

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.

Original languageEnglish (US)
Pages (from-to)226-230
Number of pages5
JournalBrain Research
Volume903
Issue number1-2
DOIs
StatePublished - Jun 8 2001
Externally publishedYes

Keywords

  • Cholinergic
  • Heat shock
  • Oxidative stress
  • Presenilin
  • Signaling

ASJC Scopus subject areas

  • Neuroscience(all)

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