Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1

Patrizia De Sarno, Mathieu Lesort, Gautam N. Bijur, Gail V W Johnson, Richard S Jope

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.

Original languageEnglish
Pages (from-to)226-230
Number of pages5
JournalBrain Research
Volume903
Issue number1-2
DOIs
StatePublished - Jun 8 2001
Externally publishedYes

Fingerprint

Presenilin-1
Cholinergic Agents
Shock
Hot Temperature
Transcription Factor AP-1
HSP70 Heat-Shock Proteins
DNA
Muscarinic Receptors
Neuroblastoma
Signal Transduction
Cell Line

Keywords

  • Cholinergic
  • Heat shock
  • Oxidative stress
  • Presenilin
  • Signaling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1. / De Sarno, Patrizia; Lesort, Mathieu; Bijur, Gautam N.; Johnson, Gail V W; Jope, Richard S.

In: Brain Research, Vol. 903, No. 1-2, 08.06.2001, p. 226-230.

Research output: Contribution to journalArticle

De Sarno, Patrizia ; Lesort, Mathieu ; Bijur, Gautam N. ; Johnson, Gail V W ; Jope, Richard S. / Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1. In: Brain Research. 2001 ; Vol. 903, No. 1-2. pp. 226-230.
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