Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1

Patrizia De Sarno; Mathieu Lesort; Gautam N. Bijur; Gail V.W. Johnson; Richard S. Jope

doi:10.1016/S0006-8993(01)02428-3

Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1

Patrizia De Sarno, Mathieu Lesort, Gautam N. Bijur, Gail V.W. Johnson, Richard S. Jope

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H₂O₂ also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.

Original language	English (US)
Pages (from-to)	226-230
Number of pages	5
Journal	Brain research
Volume	903
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(01)02428-3
State	Published - Jun 8 2001
Externally published	Yes

Keywords

Cholinergic
Heat shock
Oxidative stress
Presenilin
Signaling

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/S0006-8993(01)02428-3

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@article{9949b77f14474383918977a15ced286a,

title = "Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1",

abstract = "This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.",

keywords = "Cholinergic, Heat shock, Oxidative stress, Presenilin, Signaling",

author = "{De Sarno}, Patrizia and Mathieu Lesort and Bijur, {Gautam N.} and Johnson, {Gail V.W.} and Jope, {Richard S.}",

note = "Funding Information: We thank Dr B. Sopher (University of Washington, Seattle) for kindly providing the presenilin constructs and Dr J. Lah (Emory University, Atlanta) for presenilin antibodies. Supported by NIH Grants AG06569 and NS37768.",

year = "2001",

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doi = "10.1016/S0006-8993(01)02428-3",

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T1 - Cholinergic- and stress-induced signaling activities in cells overexpressing wild-type and mutant presenilin-1

AU - De Sarno, Patrizia

AU - Lesort, Mathieu

AU - Bijur, Gautam N.

AU - Johnson, Gail V.W.

AU - Jope, Richard S.

N1 - Funding Information: We thank Dr B. Sopher (University of Washington, Seattle) for kindly providing the presenilin constructs and Dr J. Lah (Emory University, Atlanta) for presenilin antibodies. Supported by NIH Grants AG06569 and NS37768.

PY - 2001/6/8

Y1 - 2001/6/8

N2 - This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.

AB - This study examined the effects of overexpression of presenilin-1 wild-type (PS1wt) or mutant L286V (PS1m) in human neuroblastoma SH-SY5Y cells on signal transduction systems. Oxotremorine-M-induced activation of AP-1 was 40-53% lower in PS1wt than control cells, and further impaired (63-76%) in PS1m cells. Heat shock (45°C) activated Akt, increased heat shock factor-1 (HSF-1) DNA binding activity, and increased levels of heat shock protein 70, and these responses were not altered by overexpression of PS1wt or PS1m. H2O2 also caused a time-dependent increase in HSF-1 DNA binding activity which was similar in all cell lines. Thus, overexpression of PS1wt reduced muscarinic receptor-mediated activation of AP-1, and PS1m overexpression caused greater inhibition, but stress-induced activation of Akt and HSF-1 was unaffected by either PS1wt or PS1m.

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