Chlorophyll Photobleaching in Pigment-Protein Complexes

Robert Carpentier; Roger M. Leblanc; Guy Bellemare

doi:10.1515/znc-1986-0307

Chlorophyll Photobleaching in Pigment-Protein Complexes

Robert Carpentier, Roger M. Leblanc, Guy Bellemare

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Pigment photobleaching was performed in thylakoid membranes of Hordeum vulgare (wild type, mutant Chlorina f2. Norfluranzon treated seedlings) and in pigment-protein complexes (CP-I and LHCP) isolated from H. vulgare and Chlamydomonas reinhardtii. Multiphasic kinetics were obtained in all of the above cases. Energy transfer towards pigments absorbing at longer wavelength is postulated as a general protection mechanism against photobleaching. This mechanism explains a substantial bleaching of carotenoids and a faster bleaching of chlorophyll aggregates, absorbing at long wavelength. These conclusions were valid for isolated complexes as well as for thylakoid membranes, although membranes were less sensitive to light.

Original language	English (US)
Pages (from-to)	284-290
Number of pages	7
Journal	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume	41
Issue number	3
DOIs	https://doi.org/10.1515/znc-1986-0307
State	Published - Mar 1 1986
Externally published	Yes

Keywords

Chlorophyll
Energy Transfer
Photobleaching
Pigment-Protein Complexes
Thylakoid Membranes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1515/znc-1986-0307

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title = "Chlorophyll Photobleaching in Pigment-Protein Complexes",

abstract = "Pigment photobleaching was performed in thylakoid membranes of Hordeum vulgare (wild type, mutant Chlorina f2. Norfluranzon treated seedlings) and in pigment-protein complexes (CP-I and LHCP) isolated from H. vulgare and Chlamydomonas reinhardtii. Multiphasic kinetics were obtained in all of the above cases. Energy transfer towards pigments absorbing at longer wavelength is postulated as a general protection mechanism against photobleaching. This mechanism explains a substantial bleaching of carotenoids and a faster bleaching of chlorophyll aggregates, absorbing at long wavelength. These conclusions were valid for isolated complexes as well as for thylakoid membranes, although membranes were less sensitive to light.",

keywords = "Chlorophyll, Energy Transfer, Photobleaching, Pigment-Protein Complexes, Thylakoid Membranes",

author = "Robert Carpentier and Leblanc, {Roger M.} and Guy Bellemare",

note = "Funding Information: We thank Prof. D. von Wettstein for providing seeds of the chlorina f2 mutant. Norflurazon was a kind gift from Sandoz Wander Inc. This work was supported in part by Natural Sciences and Engineering Research Council of Canada (NSERC), grants A6923 and A6358 to G. B. and R. M. L., respectively. R. C. was recipient of a NSERC fellowship.",

year = "1986",

month = mar,

day = "1",

doi = "10.1515/znc-1986-0307",

language = "English (US)",

volume = "41",

pages = "284--290",

journal = "Zeitschrift fur Naturforschung. Section C: Biosciences",

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publisher = "Verlag der Zeitschrift fur Naturforschung",

number = "3",

}

TY - JOUR

T1 - Chlorophyll Photobleaching in Pigment-Protein Complexes

AU - Carpentier, Robert

AU - Leblanc, Roger M.

AU - Bellemare, Guy

N1 - Funding Information: We thank Prof. D. von Wettstein for providing seeds of the chlorina f2 mutant. Norflurazon was a kind gift from Sandoz Wander Inc. This work was supported in part by Natural Sciences and Engineering Research Council of Canada (NSERC), grants A6923 and A6358 to G. B. and R. M. L., respectively. R. C. was recipient of a NSERC fellowship.

PY - 1986/3/1

Y1 - 1986/3/1

N2 - Pigment photobleaching was performed in thylakoid membranes of Hordeum vulgare (wild type, mutant Chlorina f2. Norfluranzon treated seedlings) and in pigment-protein complexes (CP-I and LHCP) isolated from H. vulgare and Chlamydomonas reinhardtii. Multiphasic kinetics were obtained in all of the above cases. Energy transfer towards pigments absorbing at longer wavelength is postulated as a general protection mechanism against photobleaching. This mechanism explains a substantial bleaching of carotenoids and a faster bleaching of chlorophyll aggregates, absorbing at long wavelength. These conclusions were valid for isolated complexes as well as for thylakoid membranes, although membranes were less sensitive to light.

AB - Pigment photobleaching was performed in thylakoid membranes of Hordeum vulgare (wild type, mutant Chlorina f2. Norfluranzon treated seedlings) and in pigment-protein complexes (CP-I and LHCP) isolated from H. vulgare and Chlamydomonas reinhardtii. Multiphasic kinetics were obtained in all of the above cases. Energy transfer towards pigments absorbing at longer wavelength is postulated as a general protection mechanism against photobleaching. This mechanism explains a substantial bleaching of carotenoids and a faster bleaching of chlorophyll aggregates, absorbing at long wavelength. These conclusions were valid for isolated complexes as well as for thylakoid membranes, although membranes were less sensitive to light.

KW - Chlorophyll

KW - Energy Transfer

KW - Photobleaching

KW - Pigment-Protein Complexes

KW - Thylakoid Membranes

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U2 - 10.1515/znc-1986-0307

DO - 10.1515/znc-1986-0307

M3 - Article

AN - SCOPUS:0000436715

VL - 41

SP - 284

EP - 290

JO - Zeitschrift fur Naturforschung. Section C: Biosciences

JF - Zeitschrift fur Naturforschung. Section C: Biosciences

SN - 0939-5075

IS - 3

ER -

Chlorophyll Photobleaching in Pigment-Protein Complexes

Abstract

Keywords

ASJC Scopus subject areas

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