Chlorophyll a-violaxanthin interactions in monolayers at air-water interface and in Langmuir-Blodgett films

Wieslaw I. Gruszecki, Bogumil Zelent, Heidar Ali Tajmir-Riahi, Gongming Wang, Toufik Taleb, Konka Veeranjaneyulu, Roger Leblanc

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Short distance interactions of chlorophyll a with violaxanthin (Viol) were studied in monolayers at the air-water interface and in Langmuir- Blodgett films. The surface pressure-area (π-A) isotherms for Chl a, Viol, and Chl a-Viol mixtures clearly indicate the formation of two types of complexes between Chl a and Viol with the stoichiometric ratios of 2:1 and 1:1. The complexes formed between Chl a and Viol are reflected by the changes in molecular organization of Viol in monolayers. As the fluorescence properties of Chl a molecules are known to be strongly dependent on changes in the degree of its aggregation, we followed the changes in fluorescence at different molar ratios. The molecular aggregations are noticed by the changes in band intensities at 683 and 730 nm in fluorescence emission spectra and an increase in the relative intensity of fluorescence short lifetime component of Chl a. Chl a-Viol interactions in monolayers were also made clear by the analysis of the shortwave region of the photoacoustic spectra explaining very efficient singlet-singlet excitation energy transfer from carotenoid to Chl a. Further, Chl a-Viol interactions and modeling of the formation of the complexes were also discussed based on the FTIR spectra of pure components as well as mixed systems. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume19
Issue number2
DOIs
StatePublished - Dec 15 2000

Fingerprint

Langmuir Blodgett films
chlorophylls
Chlorophyll
Langmuir-Blodgett films
Monolayers
Fluorescence
Air
fluorescence
Water
air
water
Agglomeration
interactions
carotenoids
Photoacoustic effect
Excitation energy
Energy transfer
Isotherms
isotherms
emission spectra

Keywords

  • Carotenoids
  • Chlorophyll a
  • Energy transfer
  • Fluorescence spectroscopy
  • FTIR

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Chlorophyll a-violaxanthin interactions in monolayers at air-water interface and in Langmuir-Blodgett films. / Gruszecki, Wieslaw I.; Zelent, Bogumil; Tajmir-Riahi, Heidar Ali; Wang, Gongming; Taleb, Toufik; Veeranjaneyulu, Konka; Leblanc, Roger.

In: Colloids and Surfaces B: Biointerfaces, Vol. 19, No. 2, 15.12.2000, p. 117-125.

Research output: Contribution to journalArticle

Gruszecki, Wieslaw I. ; Zelent, Bogumil ; Tajmir-Riahi, Heidar Ali ; Wang, Gongming ; Taleb, Toufik ; Veeranjaneyulu, Konka ; Leblanc, Roger. / Chlorophyll a-violaxanthin interactions in monolayers at air-water interface and in Langmuir-Blodgett films. In: Colloids and Surfaces B: Biointerfaces. 2000 ; Vol. 19, No. 2. pp. 117-125.
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