Chloramphenicol and bone marrow mitochondria

Orlando J. Martelo, David R. Manyan, Una S. Smith, Adel A Yunis

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The mechanism of bone marrow depression from chloramphenicol (CAP) remains unknown. Although CAP is a specific inhibitor of ribosomal protein synthesis in sensitive bacteria, it has little effect on ribosomal protein synthesis in mammalian cells. Recent investigations have established that mitochondria isolated from various mammalian tissues are capable of independent protein synthesis and that this process is sensitive to small concentrations of CAP. Thus interference with mitochondrial function may be a major underlying mechanism in CAP toxicity. To test this hypothesis, a study has been made on the effect of CAP on mitochondria isolated from rabbit and human bone marrow. Chloramphenicol in concentrations within therapeutic range inhibited mitochondrial protein synthesis without affecting mitochondrial respiration or oxidative phosphorylation. Evidence relating this in vitro effect of CAP to its myelotoxicity has been derived from: (1) a comparative study with antibiotics not known to be myelotoxic, and (2) electron microscopic observations on bone marrow cells from patients receiving CAP. The data lend strong support to the hypothesis that inhibition of mitochondrial protein synthesis is the biochemical basis for reversible bone marrow depression from CAP.

Original languageEnglish (US)
Pages (from-to)927-940
Number of pages14
JournalThe Journal of laboratory and clinical medicine
Volume74
Issue number6
StatePublished - Dec 1969

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Mitochondria
Chloramphenicol
Bone
Bone Marrow
Ribosomal Proteins
Mitochondrial Proteins
Protein Biosynthesis
Cells
Oxidative Phosphorylation
Bone Marrow Cells
Toxicity
Bacteria
Respiration
Electrons
Tissue
Anti-Bacterial Agents
Rabbits

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Martelo, O. J., Manyan, D. R., Smith, U. S., & Yunis, A. A. (1969). Chloramphenicol and bone marrow mitochondria. The Journal of laboratory and clinical medicine, 74(6), 927-940.

Chloramphenicol and bone marrow mitochondria. / Martelo, Orlando J.; Manyan, David R.; Smith, Una S.; Yunis, Adel A.

In: The Journal of laboratory and clinical medicine, Vol. 74, No. 6, 12.1969, p. 927-940.

Research output: Contribution to journalArticle

Martelo, OJ, Manyan, DR, Smith, US & Yunis, AA 1969, 'Chloramphenicol and bone marrow mitochondria', The Journal of laboratory and clinical medicine, vol. 74, no. 6, pp. 927-940.
Martelo OJ, Manyan DR, Smith US, Yunis AA. Chloramphenicol and bone marrow mitochondria. The Journal of laboratory and clinical medicine. 1969 Dec;74(6):927-940.
Martelo, Orlando J. ; Manyan, David R. ; Smith, Una S. ; Yunis, Adel A. / Chloramphenicol and bone marrow mitochondria. In: The Journal of laboratory and clinical medicine. 1969 ; Vol. 74, No. 6. pp. 927-940.
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