Chicken gizzard: Relation between calcium-activated phosphorylation and contraction

Phyllis E. Hoar; W. Glenn L. Kerrick; Paul S. Cassidy

doi:10.1126/science.432654

Chicken gizzard: Relation between calcium-activated phosphorylation and contraction

Phyllis E. Hoar, W. Glenn L. Kerrick, Paul S. Cassidy

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Abstract

Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large (a²⁺- and Sr²⁺-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca²⁺- and Sr²⁺-activated tensions. Adnosine 5'-O (3'- thiotriphosphate) only in the presence of Ca²⁺ induced irreversible Ca²⁺-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of ³²P from [γ-³²P]adenosine triphosphate into the myosin light chains.

Original language	English (US)
Pages (from-to)	503-506
Number of pages	4
Journal	Science
Volume	204
Issue number	4392
DOIs	https://doi.org/10.1126/science.432654
State	Published - Jan 1 1979
Externally published	Yes

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abstract = "Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large (a2+- and Sr2+-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca2+- and Sr2+-activated tensions. Adnosine 5'-O (3'- thiotriphosphate) only in the presence of Ca2+ induced irreversible Ca2+-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of 32P from [γ-32P]adenosine triphosphate into the myosin light chains.",

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AU - Kerrick, W. Glenn L.

AU - Cassidy, Paul S.

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N2 - Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large (a2+- and Sr2+-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca2+- and Sr2+-activated tensions. Adnosine 5'-O (3'- thiotriphosphate) only in the presence of Ca2+ induced irreversible Ca2+-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of 32P from [γ-32P]adenosine triphosphate into the myosin light chains.

AB - Of the proteins in mechanically disrupted chicken gizzard fibers (no functional sarcolemma) only the 20,000-dalton light chains of myosin underwent large (a2+- and Sr2+-dependent changes in phosphorylation. Phosphorylation closely corresponded with the Ca2+- and Sr2+-activated tensions. Adnosine 5'-O (3'- thiotriphosphate) only in the presence of Ca2+ induced irreversible Ca2+-insensitive activation of tension and thiophosphorylation of the 20,000-dalton light chains, and blocked incorporation of 32P from [γ-32P]adenosine triphosphate into the myosin light chains.

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Chicken gizzard: Relation between calcium-activated phosphorylation and contraction

Abstract

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