Abstract
We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
Original language | English (US) |
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Pages (from-to) | 209-216 |
Number of pages | 8 |
Journal | BioTechniques |
Volume | 46 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2009 |
Externally published | Yes |
Keywords
- Biofuel cells
- Drug discovery
- E. coli
- Host-pathogen response
- Imaging
- Microarrays
- Patterning
- Tethering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology