Abstract
We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
| Original language | English |
|---|---|
| Pages (from-to) | 209-216 |
| Number of pages | 8 |
| Journal | BioTechniques |
| Volume | 46 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2009 |
| Externally published | Yes |
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Keywords
- Biofuel cells
- Drug discovery
- E. coli
- Host-pathogen response
- Imaging
- Microarrays
- Patterning
- Tethering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
Cite this
Chemical tethering of motile bacteria to silicon surfaces. / Bearinger, Jane P.; Dugan, Lawrence C.; Wu, Ligang; Hill, Haley; Christian, Allen T.; Hubbell, Jeffrey A.
In: BioTechniques, Vol. 46, No. 3, 01.03.2009, p. 209-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical tethering of motile bacteria to silicon surfaces
AU - Bearinger, Jane P.
AU - Dugan, Lawrence C.
AU - Wu, Ligang
AU - Hill, Haley
AU - Christian, Allen T.
AU - Hubbell, Jeffrey A.
PY - 2009/3/1
Y1 - 2009/3/1
N2 - We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
AB - We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.
KW - Biofuel cells
KW - Drug discovery
KW - E. coli
KW - Host-pathogen response
KW - Imaging
KW - Microarrays
KW - Patterning
KW - Tethering
UR - http://www.scopus.com/inward/record.url?scp=65449151406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65449151406&partnerID=8YFLogxK
U2 - 10.2144/000113073
DO - 10.2144/000113073
M3 - Article
C2 - 19317664
AN - SCOPUS:65449151406
VL - 46
SP - 209
EP - 216
JO - BioTechniques
JF - BioTechniques
SN - 0736-6205
IS - 3
ER -