Chemical tethering of motile bacteria to silicon surfaces

Jane P. Bearinger, Lawrence C. Dugan, Ligang Wu, Haley Hill, Allen T. Christian, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalBioTechniques
Volume46
Issue number3
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Fingerprint

Bioelectric Energy Sources
Secondary Ion Mass Spectrometry
Biological fuel cells
Atomic Force Microscopy
Biosensing Techniques
Pathogens
Silicon
Drug Discovery
Secondary ion mass spectrometry
Carboxylic Acids
Biosensors
Immobilization
Microorganisms
Cell Wall
Escherichia coli
Amines
Atomic force microscopy
Bacteria
Cells
Imaging techniques

Keywords

  • Biofuel cells
  • Drug discovery
  • E. coli
  • Host-pathogen response
  • Imaging
  • Microarrays
  • Patterning
  • Tethering

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Cite this

Bearinger, J. P., Dugan, L. C., Wu, L., Hill, H., Christian, A. T., & Hubbell, J. A. (2009). Chemical tethering of motile bacteria to silicon surfaces. BioTechniques, 46(3), 209-216. https://doi.org/10.2144/000113073

Chemical tethering of motile bacteria to silicon surfaces. / Bearinger, Jane P.; Dugan, Lawrence C.; Wu, Ligang; Hill, Haley; Christian, Allen T.; Hubbell, Jeffrey A.

In: BioTechniques, Vol. 46, No. 3, 01.03.2009, p. 209-216.

Research output: Contribution to journalArticle

Bearinger, JP, Dugan, LC, Wu, L, Hill, H, Christian, AT & Hubbell, JA 2009, 'Chemical tethering of motile bacteria to silicon surfaces', BioTechniques, vol. 46, no. 3, pp. 209-216. https://doi.org/10.2144/000113073
Bearinger JP, Dugan LC, Wu L, Hill H, Christian AT, Hubbell JA. Chemical tethering of motile bacteria to silicon surfaces. BioTechniques. 2009 Mar 1;46(3):209-216. https://doi.org/10.2144/000113073
Bearinger, Jane P. ; Dugan, Lawrence C. ; Wu, Ligang ; Hill, Haley ; Christian, Allen T. ; Hubbell, Jeffrey A. / Chemical tethering of motile bacteria to silicon surfaces. In: BioTechniques. 2009 ; Vol. 46, No. 3. pp. 209-216.
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