Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen

An exploratory study

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Demineralization of bone for collagen extraction is most commonly performed using dilute hydrochloric acid (HCl) despite the fact that little data exists on the effects of differing acid concentration or treatment time on the quantity and quality of collagen extracts and that hydrochloric acid is extremely effective at hydrolyzing peptide bonds. Data on yield, elemental composition, and isotopic signature from bone collagen obtained from multiple extractions performed on two large ancient human bone samples from prehistoric Puerto Rico indicate that there is no meaningful difference between acid treatments ranging from 0.05 to 0.2 M concentration and 24-120 h of treatment. As such, a shorter course of demineralization using more concentrated acid would appear to produce quality results in a shorter time.

Original languageEnglish (US)
Pages (from-to)3124-3128
Number of pages5
JournalJournal of Archaeological Science
Volume37
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Puerto Rico
time
Bone Collagen
Elemental Composition
Human Bone
Signature
Collagen
Collagen Extraction

Keywords

  • δC
  • δN
  • Collagen
  • Paleodiet.
  • Radiocarbon
  • Stable isotope

ASJC Scopus subject areas

  • History
  • Archaeology

Cite this

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title = "Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: An exploratory study",
abstract = "Demineralization of bone for collagen extraction is most commonly performed using dilute hydrochloric acid (HCl) despite the fact that little data exists on the effects of differing acid concentration or treatment time on the quantity and quality of collagen extracts and that hydrochloric acid is extremely effective at hydrolyzing peptide bonds. Data on yield, elemental composition, and isotopic signature from bone collagen obtained from multiple extractions performed on two large ancient human bone samples from prehistoric Puerto Rico indicate that there is no meaningful difference between acid treatments ranging from 0.05 to 0.2 M concentration and 24-120 h of treatment. As such, a shorter course of demineralization using more concentrated acid would appear to produce quality results in a shorter time.",
keywords = "δC, δN, Collagen, Paleodiet., Radiocarbon, Stable isotope",
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AB - Demineralization of bone for collagen extraction is most commonly performed using dilute hydrochloric acid (HCl) despite the fact that little data exists on the effects of differing acid concentration or treatment time on the quantity and quality of collagen extracts and that hydrochloric acid is extremely effective at hydrolyzing peptide bonds. Data on yield, elemental composition, and isotopic signature from bone collagen obtained from multiple extractions performed on two large ancient human bone samples from prehistoric Puerto Rico indicate that there is no meaningful difference between acid treatments ranging from 0.05 to 0.2 M concentration and 24-120 h of treatment. As such, a shorter course of demineralization using more concentrated acid would appear to produce quality results in a shorter time.

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KW - Radiocarbon

KW - Stable isotope

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