Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering

Shantaram Bharadwaj, Guihua Liu, Yingai Shi, Chad Markert, Karl Erik Andersson, Anthony Atala, Yuanyuan Zhang

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Background: The goals of this study were to characterize urine-derived stem cells obtained from the upper urinary tract (uUSC), induce these cells to differentiate into urothelial and smooth muscle cells, and determine whether they could serve as a potential stem cell source for bladder tissue engineering. Materials and Methods: Urine samples were collected from five patients with normal upper urinary tracts during renal pyeloplasty. Cells were isolated from this urine and extensively expanded in vitro. Results: The mean population doubling of uUSC was 46.5±7.7. The uUSC expressed surface markers associated with mesenchymal stem cells and pericytes. These cells could differentiate into smooth muscle-like cells that expressed smooth muscle-specific gene transcripts and proteins, including α-smooth muscle actin, desmin, and myosin, when exposed to TGF-β1 and PDGF-BB. In a collagen lattice assay, these myogenic-differentiated uUSC displayed contractile function that was similar to that seen in native smooth muscle cells. Urothelial-differentiated uUSC expressed urothelial-specific genes and proteins such as uroplakin-Ia and-III, cytokeratin (CK)-7, and CK-13. Conclusions: uUSC possess expansion and differentiation (urothelial and myogenic) capabilities, and can potentially be used as an alternative cell source in bladder tissue engineering for patients needing cystoplasty.

Original languageEnglish
Pages (from-to)2123-2132
Number of pages10
JournalTissue Engineering - Part A
Volume17
Issue number15-16
DOIs
StatePublished - Aug 1 2011
Externally publishedYes

Fingerprint

Tissue Engineering
Stem cells
Urinary Tract
Tissue engineering
Muscle
Stem Cells
Urine
Smooth Muscle Myocytes
Uroplakin Ia
Uroplakin III
Urinary Bladder
Keratin-13
Smooth Muscle Myosins
Keratin-7
Pericytes
Genes
Desmin
Cells
Proteins
Mesenchymal Stromal Cells

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering. / Bharadwaj, Shantaram; Liu, Guihua; Shi, Yingai; Markert, Chad; Andersson, Karl Erik; Atala, Anthony; Zhang, Yuanyuan.

In: Tissue Engineering - Part A, Vol. 17, No. 15-16, 01.08.2011, p. 2123-2132.

Research output: Contribution to journalArticle

Bharadwaj, Shantaram ; Liu, Guihua ; Shi, Yingai ; Markert, Chad ; Andersson, Karl Erik ; Atala, Anthony ; Zhang, Yuanyuan. / Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 15-16. pp. 2123-2132.
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