Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

Tan Ince, Aurea D. Sousa, Michelle A. Jones, J. Chuck Harrell, Elin S. Agoston, Marit Krohn, Laura M. Selfors, Wenbin Liu, Ken Chen, Mao Yong, Peter Buchwald, Bin Wang, Katherine S. Hale, Evan Cohick, Petra Sergent, Abigail Witt, Zhanna Kozhekbaeva, Sizhen Gao, Agoston T. Agoston, Melissa A. MerrittRosemary Foster, Bo R. Rueda, Christopher P. Crum, Joan S. Brugge, Gordon B. Mills

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.

Original languageEnglish (US)
Article number7419
JournalNature Communications
Volume6
DOIs
StatePublished - Jun 17 2015

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Tumor Cell Line
cultured cells
Tumors
tumors
Cells
Neoplasms
Cell Line
Ovarian Neoplasms
Culture Media
Cell Culture Techniques
culture media
phenotype
Phenotype
Cell culture
therapy
Pharmaceutical Preparations
drugs
platforms
cancer
methodology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Ince, T., Sousa, A. D., Jones, M. A., Harrell, J. C., Agoston, E. S., Krohn, M., ... Mills, G. B. (2015). Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours. Nature Communications, 6, [7419]. https://doi.org/10.1038/ncomms8419

Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours. / Ince, Tan; Sousa, Aurea D.; Jones, Michelle A.; Harrell, J. Chuck; Agoston, Elin S.; Krohn, Marit; Selfors, Laura M.; Liu, Wenbin; Chen, Ken; Yong, Mao; Buchwald, Peter; Wang, Bin; Hale, Katherine S.; Cohick, Evan; Sergent, Petra; Witt, Abigail; Kozhekbaeva, Zhanna; Gao, Sizhen; Agoston, Agoston T.; Merritt, Melissa A.; Foster, Rosemary; Rueda, Bo R.; Crum, Christopher P.; Brugge, Joan S.; Mills, Gordon B.

In: Nature Communications, Vol. 6, 7419, 17.06.2015.

Research output: Contribution to journalArticle

Ince, T, Sousa, AD, Jones, MA, Harrell, JC, Agoston, ES, Krohn, M, Selfors, LM, Liu, W, Chen, K, Yong, M, Buchwald, P, Wang, B, Hale, KS, Cohick, E, Sergent, P, Witt, A, Kozhekbaeva, Z, Gao, S, Agoston, AT, Merritt, MA, Foster, R, Rueda, BR, Crum, CP, Brugge, JS & Mills, GB 2015, 'Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours', Nature Communications, vol. 6, 7419. https://doi.org/10.1038/ncomms8419
Ince, Tan ; Sousa, Aurea D. ; Jones, Michelle A. ; Harrell, J. Chuck ; Agoston, Elin S. ; Krohn, Marit ; Selfors, Laura M. ; Liu, Wenbin ; Chen, Ken ; Yong, Mao ; Buchwald, Peter ; Wang, Bin ; Hale, Katherine S. ; Cohick, Evan ; Sergent, Petra ; Witt, Abigail ; Kozhekbaeva, Zhanna ; Gao, Sizhen ; Agoston, Agoston T. ; Merritt, Melissa A. ; Foster, Rosemary ; Rueda, Bo R. ; Crum, Christopher P. ; Brugge, Joan S. ; Mills, Gordon B. / Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours. In: Nature Communications. 2015 ; Vol. 6.
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