Characterization of troponin T dilated cardiomyopathy mutations in the fetal troponin isoform

Gayathri Venkatraman, Aldrin V. Gomes, W. Glenn L. Kerrick, James D. Potter

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The major goal of this study was to elucidate how troponin T (TnT) dilated cardiomyopathy (DCM) mutations in fetal TnT and fetal troponin affect the functional properties of the fetal heart that lead to infantile cardiomyopathy. The DCM mutations R141W and ΔK210 were created in the TnT1 isoform, the primary isoform of cardiac TnT in the embryonic heart. In addition to a different TnT isoform, a different troponin I (TnI) isoform, slow skeletal TnI (ssTnI), is the dominant isoform in the embryonic heart. In skinned fiber studies, TnT1-wild-type (WT)-treated fibers reconstituted with cardiac TnI-troponin C (TnC) or ssTnI-TnC significantly increased Ca2+ sensitivity of force development when compared with TnT3-WT-treated fibers at both pH 7.0 and pH 6.5. Porcine cardiac fibers treated with TnT1 that contained the DCM mutations (R141W and ΔK210), when reconstituted with either cardiac TnI-TnC or SsTnI·TnC, significantly decreased Ca2+ sensitivity of force development compared with TnT1-WT at both pH values. The R141W mutation, which showed no significant change in the Ca2+ sensitivity of force development in the TnT3 isoform, caused a significant decrease in the TnT1 isoform. The ΔK210 mutation caused a greater decrease in Ca2+ sensitivity and maximal isometric force development compared with the R141W mutation in both the fetal and adult TnT isoforms. When complexed with cardiac TnI·TnC or ssTnI-TnC, both TnT1 DCM mutations strongly decreased maximal actomyosin ATPase activity as compared with TnT1-WT. Our results suggest that a decrease in maximal actomyosin ATPase activity in conjunction with decreased Ca2+ sensitivity of force development may cause a severe DCM phenotype in infants with the mutations.

Original languageEnglish (US)
Pages (from-to)17584-17592
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number18
DOIs
StatePublished - May 6 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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