Characterization of tissue-derived macromolecules affecting transmitter synthesis in rat spinal cord neurons

L. M. Kaufman, S. R. Barry, J. N. Barrett

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Rat spinal cord cells maintained in neuron-rich cultures were exposed to extracts of skeletal muscle or to medium conditioned by non-neuronal cells. The conditioned media enhanced neuronal acetylcholine (ACh) synthesis, choline acetyltransferase activity, and protein synthesis, and decreased γ-aminobutyric acid (GABA) synthesis. Muscle extract prepared from newborn rats produced similar enhancements but did not depress GABA synthesis. Muscle extracts prepared from normal and denervated adult rat limbs contained relatively little activity. These results suggest that different molecular factors might mediate the effects on GABA and ACh synthesis. Gel filtration of conditioned media and muscle extracts revealed that all of these activities were confined to a macromolecular fraction with an apparent M(r) of 40,000. These tissue-derived factors affecting neuronal protein and transmitter synthesis are in turn distinct from a neuronal survival-promoting factor obtained from serum (Kaufman, L.M., and J.N. Barrett (1983) Science 220: 1394-1396).

Original languageEnglish (US)
Pages (from-to)160-166
Number of pages7
JournalJournal of Neuroscience
Volume5
Issue number1
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Neuroscience(all)

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