Characterization of the stromatolite microbiome from Little Darby Island, The Bahamas using predictive and whole shotgun metagenomic analysis

Giorgio Casaburi, Alexandrea A. Duscher, Pamela R Reid, Jamie S. Foster

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Summary: Modern stromatolites represent ideal ecosystems to understand the biological processes required for the precipitation of carbonate due to their long evolutionary history and occurrence in a wide range of habitats. However, most of the prior molecular work on stromatolites has focused on understanding the taxonomic complexity and not fully elucidating the functional capabilities of these systems. Here, we begin to characterize the microbiome associated with stromatolites of Little Darby Island, The Bahamas using predictive metagenomics of the 16S rRNA gene coupled with direct whole shotgun sequencing. The metagenomic analysis of the Little Darby stromatolites revealed many shared taxa and core pathways associated with biologically induced carbonate precipitation, suggesting functional convergence within Bahamian stromatolites. A comparison of the Little Darby stromatolites with other lithifying microbial ecosystems also revealed that although factors, such as geographic location and salinity, do drive some differences within the population, there are extensive similarities within the microbial populations. These results suggest that for stromatolite formation, 'who' is in the community is not as critical as metabolic activities and environmental interactions. Together, these analyses help improve our understanding of the similarities among lithifying ecosystems and provide an important first step in characterizing the shared microbiome of modern stromatolites.

Original languageEnglish (US)
JournalEnvironmental Microbiology
DOIs
StateAccepted/In press - 2015

Fingerprint

Bahamas
Metagenomics
stromatolite
Microbiota
Firearms
Islands
carbonates
Ecosystem
ecosystems
ecosystem
microbial ecology
Carbonates
carbonate
sequence analysis
ribosomal RNA
salinity
Biological Phenomena
biological processes
Geographic Locations
history

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Characterization of the stromatolite microbiome from Little Darby Island, The Bahamas using predictive and whole shotgun metagenomic analysis. / Casaburi, Giorgio; Duscher, Alexandrea A.; Reid, Pamela R; Foster, Jamie S.

In: Environmental Microbiology, 2015.

Research output: Contribution to journalArticle

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