Characterization of the statin-like S1 and rat elongation factor 1α as two distinctly expressed messages in rat

D. K. Ann, H. H. Lin, S. Lee, Z. J. Tu, E. Wang

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Previously, we reported a rat S1 protein that is antigenically related to statin, a nonproliferating cell-specific marker; however, it shares high homology with the known human elongation factor-1α (EF-1α). To differentiate S1 from rat EF-1α and to study their respective regulation for expression, a rat EF-1α cDNA clone was isolated and characterized. The nucleotide and deduced amino acid sequences of this partial rat EF-1α cDNA are compared with that of human and mouse as well as with rat S1. Both their messages were detected in rat brain by EF-1α- or S1-specific probes. However, the mRNA encoding EF-1α is more abundant than that encoding S1. S1 and EF-1α expression were investigated in the parotid and submandibular glands of untreated rats and those treated with isoproterenol, a proliferation-inducing catecholamine. Quantitative solution hybridization demonstrated a dramatic reduction (~68%) in the S1 mRNA following isoproterenol injection in proliferation-responsive parotid glands and a mild reduction (~20%) of S1 steady-state messages in the proliferation-refractile submandibular glands. A slight increase or no changes of EF-1α levels in both parotid and submandibular glands following isoproterenol treatment are also observed. Therefore, the EF-1α and S1 genes are different genes, both expressed and regulated in vivo, but in differential quantitative and qualitative patterns.

Original languageEnglish (US)
Pages (from-to)699-702
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number2
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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