Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice

Roberto I Vazquez-Padron, L. Moreno-Fierros, L. Neri-Bazán, A. F. Martínez-Gil, G. A. De-la-Riva, R. López-Revilla

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The present paper describes important features of the immune response induced by the Cry1Ac protein from Bacillus thuringiensis in mice. The kinetics of induction of serum and mucosal antibodies showed an immediate production of anti-Cry1Ac IgM and IgG antibodies in serum after the first immunization with the protoxin by either the intraperitoneal or intragastric route. The antibody fraction in serum and intestinal fluids consisted mainly of IgG1. In addition, plasma cells producing anti-Cry1Ac IgG antibodies in Peyer's patches were observed using the solid-phase enzyme-linked immunospot (ELISPOT). Cry1Ac toxin administration induced a strong immune response in serum but in the small intestinal fluids only anti-Cry1Ac IgA antibodies were detected. The data obtained in the present study confirm that the Cry1Ac protoxin is a potent immunogen able to induce a specific immune response in the mucosal tissue, which has not been observed in response to most other proteins.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalBrazilian Journal of Medical and Biological Research
Volume33
Issue number2
StatePublished - Feb 1 2000
Externally publishedYes

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Mucosal Immunity
Bacillus thuringiensis
immune response
antibodies
Antibodies
mice
Immunoglobulin G
Serum
proteins
Immunization
mucosal immunity
Peyer's Patches
Peyer's patches
Fluids
plasma cells
Plasma Cells
Immunoglobulin A
Immunoglobulin M
immunization
Mucous Membrane

Keywords

  • Bacillus thuringiensis
  • Cry proteins
  • Mucosal immunology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice. / Vazquez-Padron, Roberto I; Moreno-Fierros, L.; Neri-Bazán, L.; Martínez-Gil, A. F.; De-la-Riva, G. A.; López-Revilla, R.

In: Brazilian Journal of Medical and Biological Research, Vol. 33, No. 2, 01.02.2000, p. 147-155.

Research output: Contribution to journalArticle

Vazquez-Padron, Roberto I ; Moreno-Fierros, L. ; Neri-Bazán, L. ; Martínez-Gil, A. F. ; De-la-Riva, G. A. ; López-Revilla, R. / Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice. In: Brazilian Journal of Medical and Biological Research. 2000 ; Vol. 33, No. 2. pp. 147-155.
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