Characterization of the Ca2+-regulatory complex of chick embryonic muscles: Polymorphism of tropomyosin in adult and embryonic fibers

Raman K. Roy, James D. Potter, Satyapriya Sarkar

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Abstract

The Ca2+-regulatory tropomyosin-troponin complex was purified from chick embryonic muscles by a combination of DEAE-cellulose chromatography and (NH4)2SO4 fractionation. The embryonic complex was very similar to that obtained from adult chicken muscles with respect to stoichiometry of components and biological activity. Tropomyosin of embryonic skeletal muscles contains both α and β subunits, the β form being the major species. In the adult stage the β form is decreased with a concomitant increase in the α form. These results indicate that i) the Ca2+-regulatory proteins are not deficient in early embryonic muscles as previously thought (Hitchcock, S.E., Develop. Biol. 23, 399, 1970), and ii) different structural genes coding for tropomyosin subunits are expressed differentially in embryonic and adult muscle fibers.

Original languageEnglish
Pages (from-to)28-36
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume70
Issue number1
DOIs
StatePublished - May 3 1976
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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