Characterization of the altered form of the c-src gene product in neuronal cells.

J. Brugge, P. Cotton, A. Lustig, W. Yonemoto, L. Lipsich, P. Coussens, J. N. Barrett, D. Nonner, R. W. Keane

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

The pp60c-src protein that is expressed at high levels in cultures of neurons from rat embryos displays an altered mobility on SDS-polyacrylamide gels due to a structural difference in the amino-terminal region of the molecule. In this report we show that the expression of this unique form of pp60c-src, designated pp60c-src(+), is not restricted to cultured neuronal cells since the pp60c-src molecules expressed in tissues from avian and rat neural tissues also display a retarded electrophoretic mobility. The amino-terminal region from pp60c-src(+) was found to contain a novel phosphorylated tryptic peptide that contains phosphoserine. However, this phosphorylation does not appear to be responsible for the retarded electrophoretic mobility of pp60c-src(+), since the mobility of this protein is not altered by phosphatase treatment under conditions that remove greater than 95% of the radiolabeled phosphate on pp60c-src(+). The altered electrophoretic form of pp60c-src was also shown to be radiolabeled with [3H]myristate, indicating that pp60c-src is fatty-acylated in neurons, as is pp60c-src in fibroblasts. The pp60c-src molecules synthesized in vitro using rabbit reticulocyte lysates programmed with mRNA from embryonic brain migrated more slowly on SDS-polyacrylamide gels than the pp60c-src protein that was translated in vitro using RNA from embryonic limb tissue. These results suggest the possibility that the c-src mRNA expressed in neurons may undergo a unique form of processing to generate the structurally distinct form of neuronal pp60c-src(+).

Original languageEnglish (US)
Pages (from-to)287-296
Number of pages10
JournalGenes & development
Volume1
Issue number3
DOIs
StatePublished - May 1987
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Brugge, J., Cotton, P., Lustig, A., Yonemoto, W., Lipsich, L., Coussens, P., Barrett, J. N., Nonner, D., & Keane, R. W. (1987). Characterization of the altered form of the c-src gene product in neuronal cells. Genes & development, 1(3), 287-296. https://doi.org/10.1101/gad.1.3.287