Characterization of the 5'-flanking region of the gene encoding the 50 kDa subunit of human DNA polymerase δ

Aymee Perez, Argentina Leon, Marietta Y.W.T. Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

DNA polymerase δ consists of at least four subunits: p125, p68, p50, and p12 [Liu et al., J. Biol. Chem. 275 (2000) 18739-18744]. We have isolated genomic DNA clones covering the gene for the human DNA polymerase δ 50 kDa subunit (POLD2) and its 5'-flanking sequence. The POLD2 gene is composed of 11 exons and is distributed over 10 kb of genomic DNA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. The 5'-flanking region of human POLD2 is G + C-rich and does not have a typical TATA box. A computer-based search for potential transcription factor binding sites revealed the existence of a number of motifs including those for AP1, AP2, Sp1, NF-1 and CREB. The functional activity of the regulatory region of the human POLD2 gene was demonstrated by its ability to drive the expression of a chloramphenicol acetyltransferase reporter gene in COS-7 cells. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1493
Issue number1-2
DOIs
StatePublished - Sep 7 2000

Keywords

  • DNA polymerase δ
  • POLD2 promoter

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

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