Characterization of serine/threonine protein phosphatases in RINm5F insulinoma cells

Åke Sjöhom, Richard E. Honkanen, Per Olof Berggren

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study investigates the occurrence and regulation of serine/threonine protein phosphatases (PPases) in insulin-secreting RINm5F insulinoma cells. PPases types 1 and 2A were identified in crude RINm5F cell homogenates by both enzymatic assay and Western blot analysis. We then characterized and compared the inhibitory actions of several compounds isolated from cyanobacteria, marine dinoflagellates and marine sponges, (viz. okadaic acid, microcystin-LR, calyculin-A and nodularin) cation-independent PPase activities in RINm5F cell homogenates. It was found that okadaic acid was the least potent inhibitor (IC50≈10-9M, IC100≈10-6M), while the other compounds exhibited IC50 values of ≈5·10-10 M and IC100≈ 5·10-9 M. The findings indicate that the inhibitory substances employed in this study may be used pharmacologically to investigate the role of serine/threonine PPases in RINm5F cell insulin secretion, a process that is likely to be regulated to a major extent by protein phosphorylation.

Original languageEnglish
Pages (from-to)349-358
Number of pages10
JournalBioscience Reports
Volume13
Issue number6
DOIs
StatePublished - Dec 1 1993
Externally publishedYes

Fingerprint

Okadaic Acid
Insulinoma
Phosphoprotein Phosphatases
Insulin
Protein Phosphatase 1
Protein Phosphatase 2
Phosphorylation
Cations
Assays
Dinoflagellida
Secretory Pathway
Enzyme Assays
Cyanobacteria
Porifera
Inhibitory Concentration 50
Proteins
Western Blotting
cyanoginosin LR
nodularin
calyculin A

Keywords

  • insulin secretion
  • insulinoma
  • okadaic acid
  • protein phosphatase

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Characterization of serine/threonine protein phosphatases in RINm5F insulinoma cells. / Sjöhom, Åke; Honkanen, Richard E.; Berggren, Per Olof.

In: Bioscience Reports, Vol. 13, No. 6, 01.12.1993, p. 349-358.

Research output: Contribution to journalArticle

Sjöhom, Åke ; Honkanen, Richard E. ; Berggren, Per Olof. / Characterization of serine/threonine protein phosphatases in RINm5F insulinoma cells. In: Bioscience Reports. 1993 ; Vol. 13, No. 6. pp. 349-358.
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