Characterization of semenogelin proteins in the human retina

Vera L. Bonilha, Mary E. Rayborn, Karen Shadrach, Lundwall Åke Lundwall, Johan Malm, Sanjoy K Bhattacharya, John W. Crabb, Joe G. Hollyfield

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Semenogelin I and II are the major proteins present in semen coagulum. In the present study, semenogelin I and II were detected in human RPE lysates by proteomic analysis. We further analyzed the expression of these proteins in the retinal cells in vivo and in vitro. Western blots detected semenogelin I and II in both RPE and neural retina while the vitreous contained only SgII. Cryo and paraffin sections of human retina were processed for both immunofluorescence and DAB reaction with an antibody that recognizes both forms of semenogelin proteins. Retina and RPE total lysates were evaluated for the presence of these proteins and in a human RPE cell line (D407). Both proteins were detected by western blot in human RPE and in D407 cell lysates. Immunoreactivity was detected in the ganglion cell and photoreceptor layer of the retina. Our data support the expression of semenogelin I and II in the human retina in several different compartments. Further studies towards addressing the function of these proteins in the retina are in progress.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalExperimental Eye Research
Volume83
Issue number1
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

Fingerprint

Retina
Proteins
Western Blotting
Photoreceptor Cells
Semen
Ganglia
Paraffin
Proteomics
Fluorescent Antibody Technique
seminal vesicle-specific antigen
Cell Line
Antibodies

Keywords

  • immunohistochemistry
  • proteomics
  • retina
  • RPE
  • semenogelins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Bonilha, V. L., Rayborn, M. E., Shadrach, K., Åke Lundwall, L., Malm, J., Bhattacharya, S. K., ... Hollyfield, J. G. (2006). Characterization of semenogelin proteins in the human retina. Experimental Eye Research, 83(1), 120-127. https://doi.org/10.1016/j.exer.2005.11.011

Characterization of semenogelin proteins in the human retina. / Bonilha, Vera L.; Rayborn, Mary E.; Shadrach, Karen; Åke Lundwall, Lundwall; Malm, Johan; Bhattacharya, Sanjoy K; Crabb, John W.; Hollyfield, Joe G.

In: Experimental Eye Research, Vol. 83, No. 1, 01.07.2006, p. 120-127.

Research output: Contribution to journalArticle

Bonilha, VL, Rayborn, ME, Shadrach, K, Åke Lundwall, L, Malm, J, Bhattacharya, SK, Crabb, JW & Hollyfield, JG 2006, 'Characterization of semenogelin proteins in the human retina', Experimental Eye Research, vol. 83, no. 1, pp. 120-127. https://doi.org/10.1016/j.exer.2005.11.011
Bonilha VL, Rayborn ME, Shadrach K, Åke Lundwall L, Malm J, Bhattacharya SK et al. Characterization of semenogelin proteins in the human retina. Experimental Eye Research. 2006 Jul 1;83(1):120-127. https://doi.org/10.1016/j.exer.2005.11.011
Bonilha, Vera L. ; Rayborn, Mary E. ; Shadrach, Karen ; Åke Lundwall, Lundwall ; Malm, Johan ; Bhattacharya, Sanjoy K ; Crabb, John W. ; Hollyfield, Joe G. / Characterization of semenogelin proteins in the human retina. In: Experimental Eye Research. 2006 ; Vol. 83, No. 1. pp. 120-127.
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