Characterization of Restrictions to Human Immunodeficiency Virus Type 1 Infection of Monocytes

Karine Triques, Mario Stevenson

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Tissue macrophages are an important cellular reservoir for replication of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus. In vitro, the ability of macrophages to support viral replication is differentiation dependent in that precursor monocytes are refractory to infection. There is, however, no consensus as to the exact point at which infection is restricted in monocytes. We have revisited this issue and have compared the efficiencies of early HIV-1 replication events in monocytes and in differentiated macrophages. Although virus entry in monocytes was comparable to that in differentiated macrophages, synthesis of full-length viral cDNAs was very inefficient. Relative to differentiated macrophages, monocytes contained low levels of dTTP due to low thymidine phosphorylase activity. Exogenous addition of D-thymidine increased dTTP levels to that in differentiated macrophages but did not correct the reverse transcription defect. These results point to a restriction in monocytes that is independent of reverse transcription precursors and suggest that differentiation-dependent cellular cofactors of reverse transcription are rate limiting in monocytes.

Original languageEnglish
Pages (from-to)5523-5527
Number of pages5
JournalJournal of Virology
Volume78
Issue number10
DOIs
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Human immunodeficiency virus 1
monocytes
HIV-1
Monocytes
macrophages
Macrophages
reverse transcription
Infection
infection
Reverse Transcription
thymidine
Thymidine Phosphorylase
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Virus Internalization
phosphorylase
Virus Replication
virus replication
Thymidine
Complementary DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of Restrictions to Human Immunodeficiency Virus Type 1 Infection of Monocytes. / Triques, Karine; Stevenson, Mario.

In: Journal of Virology, Vol. 78, No. 10, 01.05.2004, p. 5523-5527.

Research output: Contribution to journalArticle

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