Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

Connor A. Verheyen, Laura Morales, Joshua Sussman, Kalina Paunovska, Vita Manzoli, Noel Marysa Ziebarth, Alice Tomei

Research output: Contribution to journalArticle

Abstract

Islet transplantation within mechanically stable microcapsules offers the promise of long-term diabetes reversal without chronic immunosuppression. Reinforcing the ionically gelled network of alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures with desirable mechanical properties. This report describes the fabrication of hybrid PEG-ALG interpenetrating polymer networks and the investigation of microcapsule swelling, surface modulus, rheology, compression, and permeability. It is demonstrated that hybrid networks are more resistant to bulk swelling and compressive deformation and display improved shape recovery and long-term resilience. Interestingly, it is shown that PEG-ALG networks behave like ALG during microscale surface deformation and small amplitude shear while exhibiting similar permeability properties. The results from this report's in vitro characterization are interpreted according to viscoelastic polymer theory and provide new insight into hybrid hydrogel mechanical behavior. This new understanding of PEG-ALG mechanical performance is then linked to previous work that demonstrated the success of hybrid polymer immunoisolation devices in vivo.

Original languageEnglish (US)
Article number1800679
JournalMacromolecular Materials and Engineering
DOIs
StatePublished - Jan 1 2019

Fingerprint

Alginate
Polyethylene
Capsules
Polyethylenes
Polyethylene glycols
Hydrogels
Swelling
Polymers
Interpenetrating polymer networks
Hydrogel
Medical problems
Rheology
Display devices
alginic acid
Fabrication
Recovery
Mechanical properties

Keywords

  • interpenetrating networks
  • mechanical properties
  • microencapsulation
  • viscoelastic

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation. / Verheyen, Connor A.; Morales, Laura; Sussman, Joshua; Paunovska, Kalina; Manzoli, Vita; Ziebarth, Noel Marysa; Tomei, Alice.

In: Macromolecular Materials and Engineering, 01.01.2019.

Research output: Contribution to journalArticle

@article{da5198b456dc4ab1bb432d53cff28f3c,
title = "Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation",
abstract = "Islet transplantation within mechanically stable microcapsules offers the promise of long-term diabetes reversal without chronic immunosuppression. Reinforcing the ionically gelled network of alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures with desirable mechanical properties. This report describes the fabrication of hybrid PEG-ALG interpenetrating polymer networks and the investigation of microcapsule swelling, surface modulus, rheology, compression, and permeability. It is demonstrated that hybrid networks are more resistant to bulk swelling and compressive deformation and display improved shape recovery and long-term resilience. Interestingly, it is shown that PEG-ALG networks behave like ALG during microscale surface deformation and small amplitude shear while exhibiting similar permeability properties. The results from this report's in vitro characterization are interpreted according to viscoelastic polymer theory and provide new insight into hybrid hydrogel mechanical behavior. This new understanding of PEG-ALG mechanical performance is then linked to previous work that demonstrated the success of hybrid polymer immunoisolation devices in vivo.",
keywords = "interpenetrating networks, mechanical properties, microencapsulation, viscoelastic",
author = "Verheyen, {Connor A.} and Laura Morales and Joshua Sussman and Kalina Paunovska and Vita Manzoli and Ziebarth, {Noel Marysa} and Alice Tomei",
year = "2019",
month = "1",
day = "1",
doi = "10.1002/mame.201800679",
language = "English (US)",
journal = "Macromolecular Materials and Engineering",
issn = "1438-7492",
publisher = "Wiley-VCH Verlag",

}

TY - JOUR

T1 - Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

AU - Verheyen, Connor A.

AU - Morales, Laura

AU - Sussman, Joshua

AU - Paunovska, Kalina

AU - Manzoli, Vita

AU - Ziebarth, Noel Marysa

AU - Tomei, Alice

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Islet transplantation within mechanically stable microcapsules offers the promise of long-term diabetes reversal without chronic immunosuppression. Reinforcing the ionically gelled network of alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures with desirable mechanical properties. This report describes the fabrication of hybrid PEG-ALG interpenetrating polymer networks and the investigation of microcapsule swelling, surface modulus, rheology, compression, and permeability. It is demonstrated that hybrid networks are more resistant to bulk swelling and compressive deformation and display improved shape recovery and long-term resilience. Interestingly, it is shown that PEG-ALG networks behave like ALG during microscale surface deformation and small amplitude shear while exhibiting similar permeability properties. The results from this report's in vitro characterization are interpreted according to viscoelastic polymer theory and provide new insight into hybrid hydrogel mechanical behavior. This new understanding of PEG-ALG mechanical performance is then linked to previous work that demonstrated the success of hybrid polymer immunoisolation devices in vivo.

AB - Islet transplantation within mechanically stable microcapsules offers the promise of long-term diabetes reversal without chronic immunosuppression. Reinforcing the ionically gelled network of alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures with desirable mechanical properties. This report describes the fabrication of hybrid PEG-ALG interpenetrating polymer networks and the investigation of microcapsule swelling, surface modulus, rheology, compression, and permeability. It is demonstrated that hybrid networks are more resistant to bulk swelling and compressive deformation and display improved shape recovery and long-term resilience. Interestingly, it is shown that PEG-ALG networks behave like ALG during microscale surface deformation and small amplitude shear while exhibiting similar permeability properties. The results from this report's in vitro characterization are interpreted according to viscoelastic polymer theory and provide new insight into hybrid hydrogel mechanical behavior. This new understanding of PEG-ALG mechanical performance is then linked to previous work that demonstrated the success of hybrid polymer immunoisolation devices in vivo.

KW - interpenetrating networks

KW - mechanical properties

KW - microencapsulation

KW - viscoelastic

UR - http://www.scopus.com/inward/record.url?scp=85061297535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061297535&partnerID=8YFLogxK

U2 - 10.1002/mame.201800679

DO - 10.1002/mame.201800679

M3 - Article

JO - Macromolecular Materials and Engineering

JF - Macromolecular Materials and Engineering

SN - 1438-7492

M1 - 1800679

ER -