Characterization of neuropeptide Y (NPY) receptors in human hippocampus

Wei Li, Terry D. Hexum

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We identified a 50 kDa neuropeptide Y (NPY) receptor from human hippocampus by affinity labeling. NPY specific binding and labeling of the receptor were inhibited in parallel by increasing concentrations of unlabeled NPY (IC = 0.27 nM and 0.18 nM, respectively). Peptide YY (PYY), but none of the pancreatic polypeptides, was as effective as NPY in displacing [125I]NPY. NPY fragments inhibited binding with the rank order of potency: NPY > NPY13-36 > NPY20-36≥NPY18-36 > NPY1-36 free acid≥NPY26-36. These results demonstrate that the human hippocampal NPY receptor is a 50 kDa protein fitting the classification of a Y2 receptor subtype.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalBrain Research
Volume553
Issue number1
DOIs
StatePublished - Jul 5 1991
Externally publishedYes

Keywords

  • Affinity labeling
  • Hippocampus
  • Human
  • Neuropeptide Y (NPY)
  • Receptor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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