Characterization of neuritic outgrowth-promoting activity of conditioned medium on spinal cord explants

Lori B. Dribin, John N. Barrett

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Conditioned medium (CM) from muscle or fibroblast cultures dramatically increases the outgrowth of neurites from fetal rat spinal cord slices in vitro. The factor(s) in CM responsible for this enhanced outgrowth is chymotrypsin-sensitive, but neuraminidase-insensitive. At neutral pH, the factor(s) binds to a concanavalin A-agarose affinity column, a zinc metal chelate affinity column, a DE-52 anion exchange column, but not to a carboxymethyl-52 cation exchange column. These results suggest that the active CM factor(s) is a glycoprotein that is negatively charged at neutral pH. Following gel chromatography the major peak of outgrowth-promoting activity elutes at a molecular weight of approximately 50,000 daltons.

Original languageEnglish (US)
Pages (from-to)435-441
Number of pages7
JournalDevelopmental Brain Research
Volume4
Issue number4
DOIs
StatePublished - Aug 1982
Externally publishedYes

Keywords

  • SC neuritic outgrowth
  • characterization of CM factor
  • conditioned medium

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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