Characterization of neuritic outgrowth-promoting activity of conditioned medium on spinal cord explants.

L. B. Dribin, John Barrett

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Conditioned medium (CM) from muscle or fibroblast cultures dramatically increases the outgrowth of neurites from fetal rat spinal cord slices in vitro. The factor(s) in CM responsible for this enhanced outgrowth is chymotrypsin-sensitive, but neuraminidase-insensitive. At neutral pH, the factor(s) binds to a concanavalin A-agarose affinity column, a zinc metal chelate affinity column, a DE-52 anion exchange column, but not to a carboxymethyl-52 cation exchange column. These results suggest that the active CM factor(s) is a glycoprotein that is negatively charged at neutral pH. Following gel chromatography the major peak of outgrowth-promoting activity elutes at a molecular weight of approximately 50,000 daltons.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalBrain Research
Volume256
Issue number4
StatePublished - Aug 1 1982
Externally publishedYes

Fingerprint

Conditioned Culture Medium
Spinal Cord
Chymotrypsin
Neuraminidase
Concanavalin A
Sepharose
Gel Chromatography
Anions
Cations
Zinc
Glycoproteins
Fibroblasts
Molecular Weight
Metals
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of neuritic outgrowth-promoting activity of conditioned medium on spinal cord explants. / Dribin, L. B.; Barrett, John.

In: Brain Research, Vol. 256, No. 4, 01.08.1982, p. 435-441.

Research output: Contribution to journalArticle

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