Two monoclonal antibodies (MAbs) against p27 and one against p17 of simian immunodeficiency virus (SIV) from rhesus macaques were produced and characterized by reacting with disrupted, viral antigens on immunoblots. Human immunodeficiency virus type 1 (HIV-1), HIV-2 and SIV isolates from sooty mangabey, stump-tailed macaque, rhesus macaque and African green monkey (SIV(SM), SIV(StM), SIV(MAC) and SIV(AGM)) were used for comparative analysis. The p27 monoclonal antibodies HE3 and FA2 reacted with SIV(MAC) and SIV(SM), but not with HIV-1, HIV-2, SIV(StM) and SIV(AGM). The p17 monoclonal antibodies reacted with SIV(MAC) and SIV(StM), but not HIV-1, HIV-2, SIV(SM) and SIV(AGM). The differential reactivity of these monoclonal antibodies indicated that common conserved antigenic epitopes are shared between SIV(MAC) and SIV(SM) with respect to p27 MAbs and between SIV(MAC) and SIV(StM) with respect to p17. Since these MAbs reacted differently with the SIV isolates, they are useful reagents for comparative pathogenesis studies for differentiating SIV isolates.
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