Characterization of insulin regulation of lipid synthesis in MCF-7 human breast cancer cells

Marie E. Monaco, C. Kent Osborne, Thomas J. Bronzert, William R. Kidwell, Marc E. Lippman

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Stimulation of lipid synthesis by insulin in MCF-7 human breast cancer cells is characterized by an increase in acetate incorporation into long-chain fatty acids. The effects occurs in the absence of an increase in glucose uptake by the cells, and cannot be explained by a decrease in turnover of cellular fatty acids. Differential substrate experiments as well as direct measurement of enzyme activities indicate that insulin stimulates increases in activity of the first enzyme of the de novo pathway, acetyl CoA carboxylase. [3 2Pi] incorporation into phospholipids is also stimulated by insulin. Thin layer chromatography reveals five peaks of [3 2Pi]-labeled phospholipids corresponding in mobility to the following standards: lysophosphatidylcholine, sphingomyelin, phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. [3 2Pi] incorporation into each of these peaks is stimulated, although the degree of stimulation varies.

Original languageEnglish (US)
Pages (from-to)279-285
Number of pages7
JournalBreast cancer research and treatment
Volume3
Issue number3
DOIs
StatePublished - Sep 1 1983

Keywords

  • breast cancer
  • insulin
  • lipids
  • MCF-7 cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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