Characterization of infectious molecular clones of simian immunodeficiency virus (SIV(mac)) and human immunodeficiency virus type 2: Persistent infection of rhesus monkeys with molecularly cloned SIV(mac)

Y. M. Naidu, H. W. Kestler, Y. Li, C. V. Butler, D. P. Silva, D. K. Schmidt, C. D. Troup, P. K. Sehgal, P. Sonigo, M. D. Daniel, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

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Abstract

Infection of macaque monkeys with simian immunodeficiency virus (SIV) is probably the best animal model currently available for studying acquired immunodeficiency syndrome. In this report, we describe three infectious molecular clones of SIV(mac) and one of human immunodeficiency virus type 2 (HIV-2) and their use in the study of cell and species specificity, animal infection, and the relationship of gene sequence to function. Replication of the cloned viruses in different cell lines varied dramatically. Some human CD4+ cell lines (HUT 78 and MT-4) supported the replication of SIV(mac) and HIV-2, while others (CEM and Jurkat-T) supported the replication of HIV-2 but not SIV(mac). Growth of cloned virus in macaque lymphocytes in vitro was predictive of macaque infection in vivo. Macaque lymphocytes supported the replication of SIV(mac)239 and SIV(mac)251 but not SIV(mac)142 or HIV-2ROD. Using virus recovery and antibody response as criteria for infection, macaques that received cloned SIV(mac)251 and SIV(mac)239 became infected, while macaques receiving cloned SIV(mac)142 and HIV-2ROD did not become infected. Nucleotide sequences from the envelope region of all four cloned viruses demonstrated that there is considerable flexibility in the location of the translational termination (stop) signal. These infectious molecular clones will be very useful for future studies directed at the molecular basis for persistence, pathogenicity, tropism, and cell and species specificity.

Original languageEnglish (US)
Pages (from-to)4691-4696
Number of pages6
JournalJournal of Virology
Volume62
Issue number12
StatePublished - 1988
Externally publishedYes

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Human immunodeficiency virus 2
Simian immunodeficiency virus
Simian Immunodeficiency Virus
HIV-2
Macaca mulatta
Clone Cells
clones
Macaca
Infection
infection
Species Specificity
Viruses
viruses
lymphocytes
cell lines
HIV
Lymphocytes
nucleotide sequences
acquired immunodeficiency syndrome
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of infectious molecular clones of simian immunodeficiency virus (SIV(mac)) and human immunodeficiency virus type 2 : Persistent infection of rhesus monkeys with molecularly cloned SIV(mac). / Naidu, Y. M.; Kestler, H. W.; Li, Y.; Butler, C. V.; Silva, D. P.; Schmidt, D. K.; Troup, C. D.; Sehgal, P. K.; Sonigo, P.; Daniel, M. D.; Desrosiers, Ronald Charles.

In: Journal of Virology, Vol. 62, No. 12, 1988, p. 4691-4696.

Research output: Contribution to journalArticle

Naidu, Y. M. ; Kestler, H. W. ; Li, Y. ; Butler, C. V. ; Silva, D. P. ; Schmidt, D. K. ; Troup, C. D. ; Sehgal, P. K. ; Sonigo, P. ; Daniel, M. D. ; Desrosiers, Ronald Charles. / Characterization of infectious molecular clones of simian immunodeficiency virus (SIV(mac)) and human immunodeficiency virus type 2 : Persistent infection of rhesus monkeys with molecularly cloned SIV(mac). In: Journal of Virology. 1988 ; Vol. 62, No. 12. pp. 4691-4696.
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abstract = "Infection of macaque monkeys with simian immunodeficiency virus (SIV) is probably the best animal model currently available for studying acquired immunodeficiency syndrome. In this report, we describe three infectious molecular clones of SIV(mac) and one of human immunodeficiency virus type 2 (HIV-2) and their use in the study of cell and species specificity, animal infection, and the relationship of gene sequence to function. Replication of the cloned viruses in different cell lines varied dramatically. Some human CD4+ cell lines (HUT 78 and MT-4) supported the replication of SIV(mac) and HIV-2, while others (CEM and Jurkat-T) supported the replication of HIV-2 but not SIV(mac). Growth of cloned virus in macaque lymphocytes in vitro was predictive of macaque infection in vivo. Macaque lymphocytes supported the replication of SIV(mac)239 and SIV(mac)251 but not SIV(mac)142 or HIV-2ROD. Using virus recovery and antibody response as criteria for infection, macaques that received cloned SIV(mac)251 and SIV(mac)239 became infected, while macaques receiving cloned SIV(mac)142 and HIV-2ROD did not become infected. Nucleotide sequences from the envelope region of all four cloned viruses demonstrated that there is considerable flexibility in the location of the translational termination (stop) signal. These infectious molecular clones will be very useful for future studies directed at the molecular basis for persistence, pathogenicity, tropism, and cell and species specificity.",
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AU - Kestler, H. W.

AU - Li, Y.

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