Characterization of infections of human leukocytes by non-polio enteroviruses

Jenie Yoonoo Hwang, Eun Jung Jun, Ilseon Seo, Minna Won, Jeonghyun Ahn, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To elucidate the detailed susceptibilities of leukocytes to clinically important non-polio enteroviruses (EVs), primary monocytes and various human leukocyte cell lines were infected with coxsackievirus A24 (CVA24), coxsackievirus B3 (CVB3), and enterovirus 70 (EV70). The permissiveness was then assessed by determining virus replication and resultant cytopathic effects. Different EVs varied markedly in their ability to infect leukocyte cell lines. CVB3 replicated effectively in leukocytes of B-cell, T-cell, and monocyte origin, CVA24 in leukocytes of B-cell and monocyte origin, and EV70 in leukocytes of monocyte origin. Primary monocytes, as well as monocyte-derived U-937 cells, were permissive to all three EVs. We observed a positive correlation between cytotoxicity and active virus replication, except in CVB3-infected monocytes. U-937 cells efficiently generated CVB3 progeny virus without severe cellular damage, including cell death. Moreover, infectivity on leukocytes was not absolutely associated with the availability of viral receptors. These findings suggest that the susceptibility of human leukocytes to non-polio EVs may be responsible for virus transport during the viremic phase, particularly to secondary target organs, and that active replication of CVB3 in all human leukocyte lineages leads to greater dissemination, in agreement with the ability of CVB to cause systemic diseases.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalIntervirology
Volume55
Issue number5
DOIs
StatePublished - May 1 2012
Externally publishedYes

Fingerprint

Enterovirus
Leukocytes
Monocytes
Infection
Human Enterovirus D
Aptitude
Virus Replication
B-Lymphocytes
Permissiveness
Viruses
Cell Line
Cell Death
T-Lymphocytes

Keywords

  • Enterovirus infection, cytopathic effects
  • Leukocyte cell lines
  • Monocytes
  • Non-polio enterovirus
  • Receptors
  • Virus replication

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Hwang, J. Y., Jun, E. J., Seo, I., Won, M., Ahn, J., Kim, Y. K., & Lee, H. (2012). Characterization of infections of human leukocytes by non-polio enteroviruses. Intervirology, 55(5), 333-341. https://doi.org/10.1159/000329987

Characterization of infections of human leukocytes by non-polio enteroviruses. / Hwang, Jenie Yoonoo; Jun, Eun Jung; Seo, Ilseon; Won, Minna; Ahn, Jeonghyun; Kim, Yoo Kyum; Lee, Heuiran.

In: Intervirology, Vol. 55, No. 5, 01.05.2012, p. 333-341.

Research output: Contribution to journalArticle

Hwang, JY, Jun, EJ, Seo, I, Won, M, Ahn, J, Kim, YK & Lee, H 2012, 'Characterization of infections of human leukocytes by non-polio enteroviruses', Intervirology, vol. 55, no. 5, pp. 333-341. https://doi.org/10.1159/000329987
Hwang, Jenie Yoonoo ; Jun, Eun Jung ; Seo, Ilseon ; Won, Minna ; Ahn, Jeonghyun ; Kim, Yoo Kyum ; Lee, Heuiran. / Characterization of infections of human leukocytes by non-polio enteroviruses. In: Intervirology. 2012 ; Vol. 55, No. 5. pp. 333-341.
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