Characterization of functional vanilloid receptors expressed by mast cells

Tamás Bíró, Marcus Maurer, Shayan Modarres, Nancy E. Lewin, Chaya Brodie, Géza Ács, Péter Ács, Ralf Paus, Peter M. Blumberg

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Capsaicin and its ultrapotent analog resiniferatoxin (RTX) act through specific vanilloid receptors on sensory neurons. The C-type receptor is coupled to 45Ca uptake, whereas the R-type is detectable by [3H]RTX binding. We describe here specific vanilloid responses in murine mast cells (MCs). In the MC lines and in bone marrow-derived mast cells, capsaicin and RTX induced 45Ca uptake similarly to that observed for cultured rat dorsal root ganglion neurons (DRGs). This response was antagonized by the antagonists capsazepine and ruthenium red. As in DRGs, pretreatment of MCs with capsaicin or RTX induced desensitization to subsequent stimulation of 45Ca uptake. The potency for desensitization by RTX in the MCs corresponded to that for 45Ca uptake, whereas in DRGs it occurred at significantly lower concentrations corresponding to that for the high-affinity [3H]RTX binding site. Consistent with this difference, in MCs we were unable to detect [3H]RTX binding. Vanilloids were noncytotoxic to the MCs, in contrast to the DRGs. Although vanilloids did not cause degranulation in MCs, in the P815 clone capsaicin evoked selective interleukin-4 release. We conclude that certain MCs possess vanilloid receptors, but only the C-type that functions as a channel. Our finding that MCs can respond directly to capsaicin necessitates a reevaluation of the in vivo pathway of inflammation in response to vanilloids.

Original languageEnglish (US)
Pages (from-to)1332-1340
Number of pages9
JournalBlood
Volume91
Issue number4
StatePublished - Feb 15 1998
Externally publishedYes

Fingerprint

TRPV Cation Channels
Mast Cells
Capsaicin
Diagnosis-Related Groups
Neurons
Ruthenium Red
resiniferatoxin
Interleukin-4
Rats
Bone
Spinal Ganglia
Sensory Receptor Cells
Binding Sites
Cells
Clone Cells
Bone Marrow
Inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Bíró, T., Maurer, M., Modarres, S., Lewin, N. E., Brodie, C., Ács, G., ... Blumberg, P. M. (1998). Characterization of functional vanilloid receptors expressed by mast cells. Blood, 91(4), 1332-1340.

Characterization of functional vanilloid receptors expressed by mast cells. / Bíró, Tamás; Maurer, Marcus; Modarres, Shayan; Lewin, Nancy E.; Brodie, Chaya; Ács, Géza; Ács, Péter; Paus, Ralf; Blumberg, Peter M.

In: Blood, Vol. 91, No. 4, 15.02.1998, p. 1332-1340.

Research output: Contribution to journalArticle

Bíró, T, Maurer, M, Modarres, S, Lewin, NE, Brodie, C, Ács, G, Ács, P, Paus, R & Blumberg, PM 1998, 'Characterization of functional vanilloid receptors expressed by mast cells', Blood, vol. 91, no. 4, pp. 1332-1340.
Bíró T, Maurer M, Modarres S, Lewin NE, Brodie C, Ács G et al. Characterization of functional vanilloid receptors expressed by mast cells. Blood. 1998 Feb 15;91(4):1332-1340.
Bíró, Tamás ; Maurer, Marcus ; Modarres, Shayan ; Lewin, Nancy E. ; Brodie, Chaya ; Ács, Géza ; Ács, Péter ; Paus, Ralf ; Blumberg, Peter M. / Characterization of functional vanilloid receptors expressed by mast cells. In: Blood. 1998 ; Vol. 91, No. 4. pp. 1332-1340.
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