Characterization of estrogen responsive transforming activity in human breast cancer cell lines

R. B. Dickson, S. E. Bates, M. E. McManaway, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

We have characterized the production of transforming growth factor (TGF) activities by five human breast cancer cell lines in culture. The presence of TGF-α and -β activity in medium conditioned by these cells was detected by induction of anchorage-independent colony formation of normal rat kidney cells in soft agar and by epidermal growth factor and TGF-β receptor competition studies. In MCF-7, an estrogen-receptor positive cell line which requires estrogen for tumorigenesis in vivo, 17(β)-estradiol induced a 2-5 fold increase in a TGF-α-like activity (apparent molecular weight, 68,000 and 30,000 by column chromatography). An estrogen induction of lower magnitude (1.5-2.5-fold) was also seen in two other estrogen responsive cell lines, ZR-75-B and T47D. TGF-β was not induced by 17(β)-estradiol in any of the three cell lines and was decreased by up to 50% of control in the MCF-7 and T47D cell lines. TGF-β was not detectable by radioreceptor assay in the ZR-75-B cell line. Two hormone-independent and highly tumorigenic cell lines were studied. The MDA-MB-231 cell line produced large amounts of both TGF-α-like (M(r) 30,000) and TGF-β activities. In the HS578T cell line, little TGF-α was detectable, while large amounts of TGF-β were produced. No simple correlations between the tumorigenicity of the cell lines in nude mice and production of either TGF activity could be demonstrated.

Original languageEnglish
Pages (from-to)1707-1713
Number of pages7
JournalCancer Research
Volume46
Issue number4
StatePublished - Jan 1 1986
Externally publishedYes

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Transforming Growth Factors
Human Activities
Estrogens
Breast Neoplasms
Cell Line
Estradiol
Radioligand Assay
Growth Factor Receptors
MCF-7 Cells
Conditioned Culture Medium
Epidermal Growth Factor
Nude Mice
Estrogen Receptors
Agar
Chromatography
Carcinogenesis
B-Lymphocytes
Molecular Weight
Hormones
Kidney

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Dickson, R. B., Bates, S. E., McManaway, M. E., & Lippman, M. E. (1986). Characterization of estrogen responsive transforming activity in human breast cancer cell lines. Cancer Research, 46(4), 1707-1713.

Characterization of estrogen responsive transforming activity in human breast cancer cell lines. / Dickson, R. B.; Bates, S. E.; McManaway, M. E.; Lippman, Marc E.

In: Cancer Research, Vol. 46, No. 4, 01.01.1986, p. 1707-1713.

Research output: Contribution to journalArticle

Dickson, RB, Bates, SE, McManaway, ME & Lippman, ME 1986, 'Characterization of estrogen responsive transforming activity in human breast cancer cell lines', Cancer Research, vol. 46, no. 4, pp. 1707-1713.
Dickson RB, Bates SE, McManaway ME, Lippman ME. Characterization of estrogen responsive transforming activity in human breast cancer cell lines. Cancer Research. 1986 Jan 1;46(4):1707-1713.
Dickson, R. B. ; Bates, S. E. ; McManaway, M. E. ; Lippman, Marc E. / Characterization of estrogen responsive transforming activity in human breast cancer cell lines. In: Cancer Research. 1986 ; Vol. 46, No. 4. pp. 1707-1713.
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