Characterization of Escherichia coli RNase PH

Karen Ost Kelly, Murray P Deutscher

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We have previously shown that the orfE gene of Escherichia coli encodes RNase PH. Here we show that the OrfE protein (purified as described in the accompanying paper) (Jensen, K. F., Andersen, J. T., and Poulsen, P. (1992) J. Biol. Chem. 267, 17147-17152) has both the degradative and synthetic activities of RNase PH. This highly purified protein was used to characterize the enzymatic and structural properties of RNase PH. The enzyme requires a divalent cation and phosphate for activity, the latter property indicating that RNase PH is exclusively a phosphorolytic enzyme. Among tRNA-type substrates, the enzyme is most active against synthetic tRNA precursors containing extra residues following the -CCA sequence, and it can act on these molecules to generate mature tRNA with amino acid acceptor activity; 3′-phosphoryl-terminated molecules are not active as substrates. The equilibrium constant for RNase PH is near unity, suggesting that at the phosphate concentration present in vivo, the enzyme would participate in RNA degradation. The synthetic reaction of RNase PH displays a nonlinear response to increasing enzyme concentrations, and this may be due to self-aggregation of the protein. Higher order multimers of RNase PH could be detected by gel filtration at higher protein concentrations and by protein cross-linking. The possible role of RNase PH in tRNA processing is discussed.

Original languageEnglish
Pages (from-to)17153-17158
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number24
StatePublished - Aug 25 1992
Externally publishedYes

Fingerprint

Escherichia coli
Transfer RNA
Enzymes
Proteins
Amino Acid-Specific Transfer RNA
Phosphates
Molecules
ribonuclease PH
RNA Precursors
Divalent Cations
Equilibrium constants
RNA Stability
Substrates
Gel Chromatography
Structural properties
Agglomeration
Genes
Gels
RNA
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kelly, K. O., & Deutscher, M. P. (1992). Characterization of Escherichia coli RNase PH. Journal of Biological Chemistry, 267(24), 17153-17158.

Characterization of Escherichia coli RNase PH. / Kelly, Karen Ost; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 267, No. 24, 25.08.1992, p. 17153-17158.

Research output: Contribution to journalArticle

Kelly, KO & Deutscher, MP 1992, 'Characterization of Escherichia coli RNase PH', Journal of Biological Chemistry, vol. 267, no. 24, pp. 17153-17158.
Kelly KO, Deutscher MP. Characterization of Escherichia coli RNase PH. Journal of Biological Chemistry. 1992 Aug 25;267(24):17153-17158.
Kelly, Karen Ost ; Deutscher, Murray P. / Characterization of Escherichia coli RNase PH. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 24. pp. 17153-17158.
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