Characterization of ectophosphatase activities in trypanosomatid parasites of plants

P. M L Dutra, Claudia D Rodrigues, A. Romeiro, L. A M Grillo, F. A. Dias, M. Attias, W. De Souza, A. H C S Lopes, J. R. Meyer-Fernandes

Research output: Contribution to journalArticle

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Abstract

In the present work ectophosphatase activities of three trypanosomatid parasites of plants were characterized using intact cells. Phytomonas francai, Phytomonas mcgheei, and Herpetomonas sp. hydrolyzed p-nitrophenylphosphate at a rate of 5.40, 7.28, and 25.58 nmol Pi/mg of protein per min, respectively. Experiments using classical inhibitors of acid phosphatases such as sodium orthovanadate (NaVO3) and sodium fluoride (NaF) showed a decrease in phosphatase activities. Lithium fluoride (LiF) and aluminum chloride (AlCl3) were also used. Although AlCl3 had no effect, LiF was able to promote a decrease in the phosphatase activities. Interestingly, the inhibition caused by LiF was enhanced by the addition of AlCl3 during the reaction, probably due to the formation of fluoroaluminate complexes. This effect was confirmed by cytochemical analysis. In this assay, electron-dense cerium phosphate deposits were visualized on the external surface of the three parasites.

Original languageEnglish
Pages (from-to)1032-1038
Number of pages7
JournalPhytopathology
Volume90
Issue number9
StatePublished - Jan 1 2000
Externally publishedYes

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Trypanosomatidae
lithium
Phytomonas
fluorides
parasites
Herpetomonas
aluminum chloride
cerium
sodium fluoride
acid phosphatase
electrons
sodium
phosphates
assays
proteins
cells

ASJC Scopus subject areas

  • Plant Science

Cite this

Dutra, P. M. L., Rodrigues, C. D., Romeiro, A., Grillo, L. A. M., Dias, F. A., Attias, M., ... Meyer-Fernandes, J. R. (2000). Characterization of ectophosphatase activities in trypanosomatid parasites of plants. Phytopathology, 90(9), 1032-1038.

Characterization of ectophosphatase activities in trypanosomatid parasites of plants. / Dutra, P. M L; Rodrigues, Claudia D; Romeiro, A.; Grillo, L. A M; Dias, F. A.; Attias, M.; De Souza, W.; Lopes, A. H C S; Meyer-Fernandes, J. R.

In: Phytopathology, Vol. 90, No. 9, 01.01.2000, p. 1032-1038.

Research output: Contribution to journalArticle

Dutra, PML, Rodrigues, CD, Romeiro, A, Grillo, LAM, Dias, FA, Attias, M, De Souza, W, Lopes, AHCS & Meyer-Fernandes, JR 2000, 'Characterization of ectophosphatase activities in trypanosomatid parasites of plants', Phytopathology, vol. 90, no. 9, pp. 1032-1038.
Dutra PML, Rodrigues CD, Romeiro A, Grillo LAM, Dias FA, Attias M et al. Characterization of ectophosphatase activities in trypanosomatid parasites of plants. Phytopathology. 2000 Jan 1;90(9):1032-1038.
Dutra, P. M L ; Rodrigues, Claudia D ; Romeiro, A. ; Grillo, L. A M ; Dias, F. A. ; Attias, M. ; De Souza, W. ; Lopes, A. H C S ; Meyer-Fernandes, J. R. / Characterization of ectophosphatase activities in trypanosomatid parasites of plants. In: Phytopathology. 2000 ; Vol. 90, No. 9. pp. 1032-1038.
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