TY - JOUR
T1 - Characterization of cultured nail matrix cells
AU - Picardo, Mauro
AU - Tosti, Antonella
AU - Marchese, Cinzia
AU - Zompetta, Claudia
AU - Torrisi, Maria Rosaria
AU - Faggioni, Alberto
AU - Cameli, Norma
PY - 1994
Y1 - 1994
N2 - Background: Cultures of epidermal cells are commonly used to study skin biology and differentiation. Recently a method to culture nail matrix cells has been established. Objective: We report the biologic characteristics of nail matrix cells in vitro compared with those of epidermal keratinocytes. Methods: Human nail matrix cells were isolated and cultured in defined medium. Electron-microscopic examination, growth rate, integrin expression and keratin synthesis pattern were evaluated. In addition, the cells were cultured in serum-containing medium. Results: Nail matrix cells appear to be larger than human epidermal keratinocytes and, at the ultrastructural level, they contain a higher euchromatin/heterochromatin ratio and a lower nucleus/cytoplasm ratio and have a higher growth rate. The synthesis of 'hard' keratins was detected at all calcium concentrations. Immunofluorescence analyses showed the expression of α2, α3, and α6 integrin subunits. When cultured in serum-containing medium, nail matrix cells produced an outgrowth of epithelium and a spontaneous migration phenomenon associated with a tendency to stratify in a semilunar area that resembles the architecture of the nail matrix. The pluristratified epithelium showed characteristic markers of nail differentiation. Conclusion: Culture of nail matrix cells may represent a useful model to study the biologic properties of nail structure, alterations in some nail diseases and the effects of drugs.
AB - Background: Cultures of epidermal cells are commonly used to study skin biology and differentiation. Recently a method to culture nail matrix cells has been established. Objective: We report the biologic characteristics of nail matrix cells in vitro compared with those of epidermal keratinocytes. Methods: Human nail matrix cells were isolated and cultured in defined medium. Electron-microscopic examination, growth rate, integrin expression and keratin synthesis pattern were evaluated. In addition, the cells were cultured in serum-containing medium. Results: Nail matrix cells appear to be larger than human epidermal keratinocytes and, at the ultrastructural level, they contain a higher euchromatin/heterochromatin ratio and a lower nucleus/cytoplasm ratio and have a higher growth rate. The synthesis of 'hard' keratins was detected at all calcium concentrations. Immunofluorescence analyses showed the expression of α2, α3, and α6 integrin subunits. When cultured in serum-containing medium, nail matrix cells produced an outgrowth of epithelium and a spontaneous migration phenomenon associated with a tendency to stratify in a semilunar area that resembles the architecture of the nail matrix. The pluristratified epithelium showed characteristic markers of nail differentiation. Conclusion: Culture of nail matrix cells may represent a useful model to study the biologic properties of nail structure, alterations in some nail diseases and the effects of drugs.
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U2 - 10.1016/S0190-9622(94)70052-4
DO - 10.1016/S0190-9622(94)70052-4
M3 - Article
C2 - 7509353
AN - SCOPUS:0028231716
VL - 30
SP - 434
EP - 440
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
SN - 0190-9622
IS - 3
ER -