Characterization of anisotropic diffusion tensor of solute in tissue by video-FRAP imaging technique

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15 Citations (Scopus)

Abstract

In this study, a new method for determination of an anisotropic diffusion tensor by a single fluorescence recovery after photobleaching (FRAP) experiment was developed. The method was based on two independent analyses of video-FRAP images: the fast Fourier transform and the Karhunen-Loève transform. Computer-simulated FRAP tests were used to evaluate the sensitivity of the method to experimental parameters, such as the initial size of the bleached spot, the choice of the frequencies used in the Fourier analysis, the orientation of the diffusion tensor, and experimental noise. The new method was also experimentally validated by determining the anisotropic diffusion tensor of fluorescein (332 Da) in bovine annulus fibrosus. The results obtained were in agreement with those reported in a previous study. Finally, the method was used to characterize fluorescein diffusion in bovine meniscus. Our findings indicate that fluorescein diffusion in bovine meniscus is anisotropic. This study provides a new tool for the determination of anisotropic diffusion tensor that could be used to investigate the correlation between the structure of biological tissues and their transport properties.

Original languageEnglish
Pages (from-to)813-823
Number of pages11
JournalAnnals of Biomedical Engineering
Volume37
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Photobleaching
Tensors
Fluorescence
Tissue
Imaging techniques
Recovery
Fourier analysis
Fast Fourier transforms
Transport properties

Keywords

  • Annulus fibrosus (AF)
  • Bovine meniscus
  • Confocal laser scanning microscopy (CLSM)
  • Fast Fourier transform (FFT)
  • Karhunen-Loève transform (KLT)
  • Multi-layer bleaching (MLB)

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

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title = "Characterization of anisotropic diffusion tensor of solute in tissue by video-FRAP imaging technique",
abstract = "In this study, a new method for determination of an anisotropic diffusion tensor by a single fluorescence recovery after photobleaching (FRAP) experiment was developed. The method was based on two independent analyses of video-FRAP images: the fast Fourier transform and the Karhunen-Lo{\`e}ve transform. Computer-simulated FRAP tests were used to evaluate the sensitivity of the method to experimental parameters, such as the initial size of the bleached spot, the choice of the frequencies used in the Fourier analysis, the orientation of the diffusion tensor, and experimental noise. The new method was also experimentally validated by determining the anisotropic diffusion tensor of fluorescein (332 Da) in bovine annulus fibrosus. The results obtained were in agreement with those reported in a previous study. Finally, the method was used to characterize fluorescein diffusion in bovine meniscus. Our findings indicate that fluorescein diffusion in bovine meniscus is anisotropic. This study provides a new tool for the determination of anisotropic diffusion tensor that could be used to investigate the correlation between the structure of biological tissues and their transport properties.",
keywords = "Annulus fibrosus (AF), Bovine meniscus, Confocal laser scanning microscopy (CLSM), Fast Fourier transform (FFT), Karhunen-Lo{\`e}ve transform (KLT), Multi-layer bleaching (MLB)",
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AU - Travascio, Francesco

AU - Zhao, Weizhao

AU - Gu, Weiyong

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N2 - In this study, a new method for determination of an anisotropic diffusion tensor by a single fluorescence recovery after photobleaching (FRAP) experiment was developed. The method was based on two independent analyses of video-FRAP images: the fast Fourier transform and the Karhunen-Loève transform. Computer-simulated FRAP tests were used to evaluate the sensitivity of the method to experimental parameters, such as the initial size of the bleached spot, the choice of the frequencies used in the Fourier analysis, the orientation of the diffusion tensor, and experimental noise. The new method was also experimentally validated by determining the anisotropic diffusion tensor of fluorescein (332 Da) in bovine annulus fibrosus. The results obtained were in agreement with those reported in a previous study. Finally, the method was used to characterize fluorescein diffusion in bovine meniscus. Our findings indicate that fluorescein diffusion in bovine meniscus is anisotropic. This study provides a new tool for the determination of anisotropic diffusion tensor that could be used to investigate the correlation between the structure of biological tissues and their transport properties.

AB - In this study, a new method for determination of an anisotropic diffusion tensor by a single fluorescence recovery after photobleaching (FRAP) experiment was developed. The method was based on two independent analyses of video-FRAP images: the fast Fourier transform and the Karhunen-Loève transform. Computer-simulated FRAP tests were used to evaluate the sensitivity of the method to experimental parameters, such as the initial size of the bleached spot, the choice of the frequencies used in the Fourier analysis, the orientation of the diffusion tensor, and experimental noise. The new method was also experimentally validated by determining the anisotropic diffusion tensor of fluorescein (332 Da) in bovine annulus fibrosus. The results obtained were in agreement with those reported in a previous study. Finally, the method was used to characterize fluorescein diffusion in bovine meniscus. Our findings indicate that fluorescein diffusion in bovine meniscus is anisotropic. This study provides a new tool for the determination of anisotropic diffusion tensor that could be used to investigate the correlation between the structure of biological tissues and their transport properties.

KW - Annulus fibrosus (AF)

KW - Bovine meniscus

KW - Confocal laser scanning microscopy (CLSM)

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KW - Karhunen-Loève transform (KLT)

KW - Multi-layer bleaching (MLB)

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