Characterization of an unique RNA initiated immediately upstream from human α1 globin gene in vivo and in vitro

Polymerase II-dependence, tissue specificity, and subcellular location

John Hess, Carlos Perez-Stable, Al Deisseroth, Che Kun James Shen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. RNAs isolated from these cell lines, however, do not contain this upstream transcript. The putative 5′ end of the α1 globin upstream RNA is mapped by primer extension to base -45, which is located in between the CCAAT and TATA boxes. The synthesis of this RNA in vitro and in vivo, and the close proximity of its 5′ end to the promoter of the α1 globin gene suggest a common mechanism regulating the transcriptional initiation of both the upstream and the major α1 globin RNAs.

Original languageEnglish
Pages (from-to)6059-6074
Number of pages16
JournalNucleic Acids Research
Volume13
Issue number17
DOIs
StatePublished - Sep 11 1985
Externally publishedYes

Fingerprint

Organ Specificity
Globins
RNA
Specificity
Immediately
Genes
Tissue
Gene
Cell
Cells
COS Cells
Cell Line
Line
Leukemia, Erythroblastic, Acute
TATA Box
RNA Polymerase II
HeLa Cells
Bone
Promoter
Bone Marrow Cells

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

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title = "Characterization of an unique RNA initiated immediately upstream from human α1 globin gene in vivo and in vitro: Polymerase II-dependence, tissue specificity, and subcellular location",
abstract = "We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. RNAs isolated from these cell lines, however, do not contain this upstream transcript. The putative 5′ end of the α1 globin upstream RNA is mapped by primer extension to base -45, which is located in between the CCAAT and TATA boxes. The synthesis of this RNA in vitro and in vivo, and the close proximity of its 5′ end to the promoter of the α1 globin gene suggest a common mechanism regulating the transcriptional initiation of both the upstream and the major α1 globin RNAs.",
author = "John Hess and Carlos Perez-Stable and Al Deisseroth and Shen, {Che Kun James}",
year = "1985",
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T1 - Characterization of an unique RNA initiated immediately upstream from human α1 globin gene in vivo and in vitro

T2 - Polymerase II-dependence, tissue specificity, and subcellular location

AU - Hess, John

AU - Perez-Stable, Carlos

AU - Deisseroth, Al

AU - Shen, Che Kun James

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N2 - We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. RNAs isolated from these cell lines, however, do not contain this upstream transcript. The putative 5′ end of the α1 globin upstream RNA is mapped by primer extension to base -45, which is located in between the CCAAT and TATA boxes. The synthesis of this RNA in vitro and in vivo, and the close proximity of its 5′ end to the promoter of the α1 globin gene suggest a common mechanism regulating the transcriptional initiation of both the upstream and the major α1 globin RNAs.

AB - We have identified an abundant transcript initiated upstream from the canonical cap site of human α1 globin gene in bone marrow cells and in COS-7 cells transfected with an α1 globin gene-containing plasmid. Similar to the major α1 globin transcript, this upstream RNA is present almost exclusively in the cytoplasm of the transfected COS-7 cells. It is also synthesized efficiently in vitro by RNA polymerase II in the nuclear extracts prepared from a Hela cell line and an erythroleukemia cell line, K562. RNAs isolated from these cell lines, however, do not contain this upstream transcript. The putative 5′ end of the α1 globin upstream RNA is mapped by primer extension to base -45, which is located in between the CCAAT and TATA boxes. The synthesis of this RNA in vitro and in vivo, and the close proximity of its 5′ end to the promoter of the α1 globin gene suggest a common mechanism regulating the transcriptional initiation of both the upstream and the major α1 globin RNAs.

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