Abstract
A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
| Original language | English |
|---|---|
| Pages (from-to) | 1362-1367 |
| Number of pages | 6 |
| Journal | Cancer Research |
| Volume | 38 |
| Issue number | 5 |
| State | Published - Dec 1 1978 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia. / Lazarus, H.; Barell, E. F.; Krishan, A.; Livingston, D. M.; Harris, K.; Schlossman, S. F.; Chess, L.
In: Cancer Research, Vol. 38, No. 5, 01.12.1978, p. 1362-1367.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia
AU - Lazarus, H.
AU - Barell, E. F.
AU - Krishan, A.
AU - Livingston, D. M.
AU - Harris, K.
AU - Schlossman, S. F.
AU - Chess, L.
PY - 1978/12/1
Y1 - 1978/12/1
N2 - A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
AB - A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018179162&partnerID=8YFLogxK
M3 - Article
C2 - 205365
AN - SCOPUS:0018179162
VL - 38
SP - 1362
EP - 1367
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 5
ER -