Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia

H. Lazarus, E. F. Barell, A. Krishan, D. M. Livingston, K. Harris, S. F. Schlossman, L. Chess

Research output: Contribution to journalArticle

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Abstract

A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.

Original languageEnglish
Pages (from-to)1362-1367
Number of pages6
JournalCancer Research
Volume38
Issue number5
StatePublished - Dec 1 1978
Externally publishedYes

Fingerprint

Null Lymphocytes
Leukemia
Lymphocytes
Cell Line
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Erythrocytes
Epstein-Barr Virus Nuclear Antigens
HLA-D Antigens
T-Lymphocytes
B-Cell Antigen Receptors
Karyotype
Immune Sera
Sheep
Glycoproteins
B-Lymphocytes
Phenotype
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lazarus, H., Barell, E. F., Krishan, A., Livingston, D. M., Harris, K., Schlossman, S. F., & Chess, L. (1978). Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia. Cancer Research, 38(5), 1362-1367.

Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia. / Lazarus, H.; Barell, E. F.; Krishan, A.; Livingston, D. M.; Harris, K.; Schlossman, S. F.; Chess, L.

In: Cancer Research, Vol. 38, No. 5, 01.12.1978, p. 1362-1367.

Research output: Contribution to journalArticle

Lazarus, H, Barell, EF, Krishan, A, Livingston, DM, Harris, K, Schlossman, SF & Chess, L 1978, 'Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia', Cancer Research, vol. 38, no. 5, pp. 1362-1367.
Lazarus H, Barell EF, Krishan A, Livingston DM, Harris K, Schlossman SF et al. Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia. Cancer Research. 1978 Dec 1;38(5):1362-1367.
Lazarus, H. ; Barell, E. F. ; Krishan, A. ; Livingston, D. M. ; Harris, K. ; Schlossman, S. F. ; Chess, L. / Characterization of a unique cell line (LAZ 221) from human acute lymphocyte ('null' cell) leukemia. In: Cancer Research. 1978 ; Vol. 38, No. 5. pp. 1362-1367.
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