TY - JOUR
T1 - Characterization of a Unique Cell Line (Laz 221) from Human Acute Lymphocytic (“Null” Cell) Leukemia
AU - Lazarus, Herbert
AU - Barell, Elizabeth F.
AU - Krishan, Awtar
AU - Livingston, David M.
AU - Harris, Kathleen
AU - Stuart, S. F.
AU - Schlossman, S.
AU - Chess, Leonard
PY - 1978/5
Y1 - 1978/5
N2 - A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
AB - A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
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M3 - Article
C2 - 205365
AN - SCOPUS:0018179162
VL - 38
SP - 1362
EP - 1367
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 5
ER -
