Characterization of a Unique Cell Line (Laz 221) from Human Acute Lymphocytic (“Null” Cell) Leukemia

Herbert Lazarus, Elizabeth F. Barell, Awtar Krishan, David M. Livingston, Kathleen Harris, S. F. Stuart, S. Schlossman, Leonard Chess

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34 Scopus citations


A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the 'null' cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12+t(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH 1 -, HTL -), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.

Original languageEnglish (US)
Pages (from-to)1362-1367
Number of pages6
JournalCancer Research
Issue number5
StatePublished - May 1978
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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