Characterization of a cyclooxygenase-2-765G→C promoter polymorphism in human neural cells

Jian Guo Cui, Shabnam Salehi-Rad, Ekaterina Rogaeva, Walter J. Lukiw

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Direct sequencing of the human cyclooxygenase-2 gene promoter revealed a common single nucleotide substitution, cyclooxygenase-2-765G→C, in 24.5% of the populations analyzed. This change introduced a 20 base pair polypyrimidine/polypurine element and a partial recognition feature for RXRα, the 9-cis retinoic acid receptor, into the polymorphic promoter. Cyclooxygenase-2-765G→C constructs, when transfected into human neural cells, exhibited a 1.4-fold higher level of basal expression, while the proinflammatory factors interleukin-1β and 9-cis retinoic acid synergistically induced polymorphic promoter activity 2.4-fold over wild type. These results suggest that under specific conditions of cellular stress, a common variation in cyclooxygenase-2 promoter structure may enhance cyclooxygenase-2 transcription, and this may contribute to the proliferation of an inflammatory response in brain cells.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalNeuroReport
Volume16
Issue number6
DOIs
StatePublished - Apr 25 2005

Fingerprint

Cyclooxygenase 2
Interleukin-9
Retinoid X Receptors
Interleukin-1
Base Pairing
Nucleotides
Brain
Population
Genes

Keywords

  • 9 cis-retinoic acid
  • Alzheimer's disease
  • Cyclooxygenase-2
  • Inflammatory response
  • Interleukin-1β
  • Neural cells
  • Promoter polymorphism

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of a cyclooxygenase-2-765G→C promoter polymorphism in human neural cells. / Cui, Jian Guo; Salehi-Rad, Shabnam; Rogaeva, Ekaterina; Lukiw, Walter J.

In: NeuroReport, Vol. 16, No. 6, 25.04.2005, p. 575-579.

Research output: Contribution to journalArticle

Cui, Jian Guo ; Salehi-Rad, Shabnam ; Rogaeva, Ekaterina ; Lukiw, Walter J. / Characterization of a cyclooxygenase-2-765G→C promoter polymorphism in human neural cells. In: NeuroReport. 2005 ; Vol. 16, No. 6. pp. 575-579.
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