Characterization of a chicken cDNA encoding the retinoblastoma gene product

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Abstract

We have isolated a chicken cDNA that encodes the retinoblastoma susceptibility gene product (RB). The predicted amino acid sequence of the chicken RB protein is highly similar to that of the mouse, human and Xenopus RB proteins in regions of known functions; however, chicken RB has distinct species-specific differences, including a shorter N-terminal region as compared to the mouse and human RB proteins. In vitro-translated chicken RB co-migrates on SDS-polyacrylamide gels with endogenous RB synthesized in transformed chicken spleen cells. Finally, chicken RB is located in the nucleus of chicken embryo fibroblasts when overexpressed from a retroviral vector.

Original languageEnglish
Pages (from-to)82-86
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1218
Issue number1
DOIs
StatePublished - May 17 1994
Externally publishedYes

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Keywords

  • Cancer gene
  • Retinoblastoma
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Feinstein, R., Bolton, W. K., Quinones, J. N., Mosialos, G., Sif, S., Huff, J. L., Capobianco, A. J., & Gilmore, T. D. (1994). Characterization of a chicken cDNA encoding the retinoblastoma gene product. BBA - Gene Structure and Expression, 1218(1), 82-86. https://doi.org/10.1016/0167-4781(94)90103-1