Abstract
Our study is to show the safety of transfusion and the number, phenotype, and proliferative potential of in vitro cultivated autologous hematopoietic peripheral blood progenitor/stem cells (PBPCs). An in vitro long-term liquid culture using PBPC suspension from consenting patients with metastatic breast cancer was established. The medium was supplemented with a variety of hematopoietic growth factors. The mononuclear cells (MNCs), their viability, CD34+ subsets, clonogenic cells, and neutrophil function were measured prior to, during, and after liquid culture for 14 days. The total cell number increased during incubation in vitro from 2.5 × 108 to 5 × 109. The clonogenic and CD34+ cells increased during the first week 6- and 3.5-fold, respectively, and were almost undetectable after 2 weeks. Maturation into the myeloid cell series was demonstrated by standard cytology and increase of CD33+ and CD38+ cell numbers. On average, 1.5 × 109 cells were transfused to consenting patients with metastatic breast cancer after high-dose chemotherapy and PBPC transplantation at nadir of WBC ≤0.1/nL. One hour later, the mean WBC was measurable at 0.3/nL. Subsequently, WBC counts dropped to 0.2/nL and 0.1/ nL at 6 and 24 h post transfusion. No side effects and complications were observed. In summary, an in vitro expansion can produce a ≥20-fold increase of maturing PBPCs for an effective and safe autologous transfusion. This unique approach, when refined, could lead to a safer post-transplant period and a decrease of complications due to neutropenic fever.
| Original language | English |
|---|---|
| Pages (from-to) | 273-281 |
| Number of pages | 9 |
| Journal | Transfusion Science |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Hematology
Cite this
Characterization and transfusion of in vitro cultivated hematopoietic progenitor cells. / Glück, Stefan; Chadderton, Tony; Porter, Kathy; Dietz, Gunter; Maruyama, Midori.
In: Transfusion Science, Vol. 16, No. 3, 01.01.1995, p. 273-281.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization and transfusion of in vitro cultivated hematopoietic progenitor cells
AU - Glück, Stefan
AU - Chadderton, Tony
AU - Porter, Kathy
AU - Dietz, Gunter
AU - Maruyama, Midori
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Our study is to show the safety of transfusion and the number, phenotype, and proliferative potential of in vitro cultivated autologous hematopoietic peripheral blood progenitor/stem cells (PBPCs). An in vitro long-term liquid culture using PBPC suspension from consenting patients with metastatic breast cancer was established. The medium was supplemented with a variety of hematopoietic growth factors. The mononuclear cells (MNCs), their viability, CD34+ subsets, clonogenic cells, and neutrophil function were measured prior to, during, and after liquid culture for 14 days. The total cell number increased during incubation in vitro from 2.5 × 108 to 5 × 109. The clonogenic and CD34+ cells increased during the first week 6- and 3.5-fold, respectively, and were almost undetectable after 2 weeks. Maturation into the myeloid cell series was demonstrated by standard cytology and increase of CD33+ and CD38+ cell numbers. On average, 1.5 × 109 cells were transfused to consenting patients with metastatic breast cancer after high-dose chemotherapy and PBPC transplantation at nadir of WBC ≤0.1/nL. One hour later, the mean WBC was measurable at 0.3/nL. Subsequently, WBC counts dropped to 0.2/nL and 0.1/ nL at 6 and 24 h post transfusion. No side effects and complications were observed. In summary, an in vitro expansion can produce a ≥20-fold increase of maturing PBPCs for an effective and safe autologous transfusion. This unique approach, when refined, could lead to a safer post-transplant period and a decrease of complications due to neutropenic fever.
AB - Our study is to show the safety of transfusion and the number, phenotype, and proliferative potential of in vitro cultivated autologous hematopoietic peripheral blood progenitor/stem cells (PBPCs). An in vitro long-term liquid culture using PBPC suspension from consenting patients with metastatic breast cancer was established. The medium was supplemented with a variety of hematopoietic growth factors. The mononuclear cells (MNCs), their viability, CD34+ subsets, clonogenic cells, and neutrophil function were measured prior to, during, and after liquid culture for 14 days. The total cell number increased during incubation in vitro from 2.5 × 108 to 5 × 109. The clonogenic and CD34+ cells increased during the first week 6- and 3.5-fold, respectively, and were almost undetectable after 2 weeks. Maturation into the myeloid cell series was demonstrated by standard cytology and increase of CD33+ and CD38+ cell numbers. On average, 1.5 × 109 cells were transfused to consenting patients with metastatic breast cancer after high-dose chemotherapy and PBPC transplantation at nadir of WBC ≤0.1/nL. One hour later, the mean WBC was measurable at 0.3/nL. Subsequently, WBC counts dropped to 0.2/nL and 0.1/ nL at 6 and 24 h post transfusion. No side effects and complications were observed. In summary, an in vitro expansion can produce a ≥20-fold increase of maturing PBPCs for an effective and safe autologous transfusion. This unique approach, when refined, could lead to a safer post-transplant period and a decrease of complications due to neutropenic fever.
UR - http://www.scopus.com/inward/record.url?scp=0028879466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028879466&partnerID=8YFLogxK
U2 - 10.1016/0955-3886(95)00035-V
DO - 10.1016/0955-3886(95)00035-V
M3 - Article
C2 - 10159885
AN - SCOPUS:0028879466
VL - 16
SP - 273
EP - 281
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
SN - 1473-0502
IS - 3
ER -