Characterization and functional analysis of the expression of intercellular adhesion molecule-1 in human papillomavirus-related disease of cervical keratinocytes

Nicholas Coleman, Isobel M. Greenfield, John Hare, Huw S Kruger Gray, Benjamin M. Chain, Margaret A. Stanley

Research output: Contribution to journalArticle

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Abstract

We bave investigated the expression of intercellular adhesion molecule-1 (ICAM-1) in squamous neoplasia of the cervix and have noted a significant induction of the molecule in high-grade intra-epitbelial lesions. Using monolayer and organotypic in vitro tissue culture systems, we have shown that there is no constitutive ICAM-1 expression on cervical keratinocytes immortalized but not transformed by human papillomavirus type 16, whereas two human papittomaviruses type 16 containing and fully transformed cervical keratinocyte lines do constitutively express the molecule. AU cell types, including human papillomavirus-negative normal cervical keratinocytes, can be induced to up-regulate their expression of ICAM-1 by pro-inflammatory cytokines such as interferon-γ. In addition, we have used an in vitro adhesion assay to show that ICAM-1:lymphocyte Junction antigen-1 interaction is functionally important in lymphocyte binding to cervical keratinocytes, suggesting a role for ICAM-1 in retaining and enabling functional activity of lymphocytes in the cervix in intraepithelial neoplasia.

Original languageEnglish (US)
Pages (from-to)355-367
Number of pages13
JournalAmerican Journal of Pathology
Volume143
Issue number2
StatePublished - Aug 1993
Externally publishedYes

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Intercellular Adhesion Molecule-1
Keratinocytes
Lymphocytes
Cervix Uteri
Human papillomavirus 16
Interferons
Neoplasms
Up-Regulation
Cytokines
Antigens
In Vitro Techniques

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Characterization and functional analysis of the expression of intercellular adhesion molecule-1 in human papillomavirus-related disease of cervical keratinocytes. / Coleman, Nicholas; Greenfield, Isobel M.; Hare, John; Kruger Gray, Huw S; Chain, Benjamin M.; Stanley, Margaret A.

In: American Journal of Pathology, Vol. 143, No. 2, 08.1993, p. 355-367.

Research output: Contribution to journalArticle

Coleman, Nicholas ; Greenfield, Isobel M. ; Hare, John ; Kruger Gray, Huw S ; Chain, Benjamin M. ; Stanley, Margaret A. / Characterization and functional analysis of the expression of intercellular adhesion molecule-1 in human papillomavirus-related disease of cervical keratinocytes. In: American Journal of Pathology. 1993 ; Vol. 143, No. 2. pp. 355-367.
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