Characterization and Distribution of Transferrin Receptors in the Rat Brain

Deborah C. Mash, John Pablo, Donna D. Flynn, Simon M.N. Efange, William J. Weiner

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Transferrin receptors were characterized with 125I-ferrotransferrin on membrane fractions prepared from the rodent forebrain. The distribution of transferrin receptors in the rat brain was investigated further by in vitro autoradiography. Saturation binding analysis revealed an apparent single class of sites with a dissociation constant of 2 nM and a binding site density of 15 pmol/g. The Hill coefficient derived from these data was 1.05, indicating the absence of cooperativity and that 125I-ferrotransferrin binds to a single class of sites. Estimates of the kinetically determined KD for forebrain membranes were within the 2-4 nM range, in agreement with the equilibrium measurements. Apotransferrin and ferrotransferrin competitively displaced the binding of 125I-ferrotransferrin, while ferritin, albumin, and cyto-chrome c failed to compete for the binding site. Ceruloplasmin, the copper transport protein, was a weak inhibitor of 125-ferrotransferrin binding. Autoradiographic localization studies demonstrate a heterogeneous distribution of transferrin receptors in the rat brain. Transferrin receptor densities were markedly elevated over the cerebral cortex and the hippocampus. Moderate to high 125I-ferrotransferrin binding was also apparent throughout areas involved in motor functions, including the caudate-putamen, the nucleus accumbens, the substantia nigra, the red nucleus, and the cerebellum.

Original languageEnglish (US)
Pages (from-to)1972-1979
Number of pages8
JournalJournal of neurochemistry
Volume55
Issue number6
DOIs
StatePublished - Dec 1990

Keywords

  • Autoradiography
  • Iron
  • Localization
  • Receptor
  • Transferrin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Characterization and Distribution of Transferrin Receptors in the Rat Brain'. Together they form a unique fingerprint.

  • Cite this

    Mash, D. C., Pablo, J., Flynn, D. D., Efange, S. M. N., & Weiner, W. J. (1990). Characterization and Distribution of Transferrin Receptors in the Rat Brain. Journal of neurochemistry, 55(6), 1972-1979. https://doi.org/10.1111/j.1471-4159.1990.tb05784.x