Characterization and changes of a chromosomal-scaffolding protein in human epithelia

Dora E. Vega-Salas, Manuel Fernandez, Keyvan Nouri

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Chromosomal-scaffolding proteins exert DNA structural functions during mitosis, and gene regulatory functions such as RNA splicing/polymerization and DNA replication in interphase, allowing the progression of the cell cycle. Recently, it has been reported that topoisomerases play a key role in DNA repair, suggesting an additional regulatory mechanism of the chromosome structure on DNA metabolism and cell cycle checkpoints. Despite the progress made toward the understanding of the genome organization and expression, few changes have been reported in the chromosome scaffold of malignant cells associated with the cancer phenotype. In a previous work, we reported LFM-1 protein (Licensing Factor Model-1) as a chromosomal-scaffold component transiently associated with mitotic chromosomes in MDCK (Madin Darby canine kidney) epithelial cells (Vega-Salas and Salas 1996). In this work, we explore LFM-1 expression in human epithelia with contrasting tumorigenicity during the progression of the cell cycle. Although cell metabolic labeling shows synthesis of a common 87-kDa LFM-1 precursor during G2-phase in both non-tumorigenic and cancer cells, surprisingly, the post-translational LFM-1 chromosome-bound polypeptide displays a different apparent molecular weight and binding to chromosomes in the cancer phenotype. The finding of a highly phosphorylated LFM-1 60-kDa form with abnormal binding to chromosomes in human carcinoma cells suggests a structural/regulatory role(s) of the chromosome-scaffold/matrix in DNA metabolism in cancer-related events of cell proliferation.

Original languageEnglish (US)
Pages (from-to)193-203
Number of pages11
JournalCell and Tissue Research
Volume308
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Cancer
  • Cell cycle
  • Chromosomal-scaffold
  • Epithelia
  • Human
  • Proliferation

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

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