Characteristics of the plasma disappearance of [3H] thyrotropin releasing hormone in the rat

A. Dupont, F. Labrie, L. Levasseur, Andrew V Schally

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Thirty seconds after an intravenous injection of 0.5 ml of 2.5 x 10-6M [3H]thyrotropin releasing hormone (TRH) to male rats, the radioactivity was distributed in an apparent volume of 134 ml, thus reflecting its rapid disappearance from the blood. The pattern of plasma radioactivity after intravenous injection of [3H]TRH could be represented by the sum of 3 exponentials, suggesting a 3 compartment model of plasma disappearance of radioactivity. The half life of TRH and of its metabolites calculated from the first exponential curve was 2.2 min. Thin layer electrophoresis of methanol extracts of plasma collected 1, 7, 20, and 30 min after injection of [3H]TRH showed that the neurohormone was transformed into TRH free acid in proportions of 58, 58, 52 and 47%, respectively. The first 60 min seemed to be the most important period for elimination of the hormone and its metabolites by the kidney. In fact, 60 min after injection, 38% of the total radioactivity was found in the bladder. At 2, 15 and 30 min after injection of [3H]TRH, high accumulation of radioactivity occurred in kidneys, pineal gland, and anterior and posterior (including intermediate) lobes of the pituitary. High levels of radioactivity were seen in kidneys and anterior pituitary gland at all time intervals studied.

Original languageEnglish
Pages (from-to)1012-1019
Number of pages8
JournalCanadian Journal of Physiology and Pharmacology
Volume52
Issue number5
StatePublished - Dec 1 1974
Externally publishedYes

Fingerprint

Thyrotropin-Releasing Hormone
Radioactivity
Kidney
Intravenous Injections
Injections
Intermediate Pituitary Gland
Anterior Pituitary Gland
Pineal Gland
Neurotransmitter Agents
Methanol
Half-Life
Electrophoresis
Urinary Bladder
Hormones
Acids

ASJC Scopus subject areas

  • Physiology
  • Pharmacology

Cite this

Characteristics of the plasma disappearance of [3H] thyrotropin releasing hormone in the rat. / Dupont, A.; Labrie, F.; Levasseur, L.; Schally, Andrew V.

In: Canadian Journal of Physiology and Pharmacology, Vol. 52, No. 5, 01.12.1974, p. 1012-1019.

Research output: Contribution to journalArticle

@article{21168c4099aa4c29a393d26f5ad28375,
title = "Characteristics of the plasma disappearance of [3H] thyrotropin releasing hormone in the rat",
abstract = "Thirty seconds after an intravenous injection of 0.5 ml of 2.5 x 10-6M [3H]thyrotropin releasing hormone (TRH) to male rats, the radioactivity was distributed in an apparent volume of 134 ml, thus reflecting its rapid disappearance from the blood. The pattern of plasma radioactivity after intravenous injection of [3H]TRH could be represented by the sum of 3 exponentials, suggesting a 3 compartment model of plasma disappearance of radioactivity. The half life of TRH and of its metabolites calculated from the first exponential curve was 2.2 min. Thin layer electrophoresis of methanol extracts of plasma collected 1, 7, 20, and 30 min after injection of [3H]TRH showed that the neurohormone was transformed into TRH free acid in proportions of 58, 58, 52 and 47{\%}, respectively. The first 60 min seemed to be the most important period for elimination of the hormone and its metabolites by the kidney. In fact, 60 min after injection, 38{\%} of the total radioactivity was found in the bladder. At 2, 15 and 30 min after injection of [3H]TRH, high accumulation of radioactivity occurred in kidneys, pineal gland, and anterior and posterior (including intermediate) lobes of the pituitary. High levels of radioactivity were seen in kidneys and anterior pituitary gland at all time intervals studied.",
author = "A. Dupont and F. Labrie and L. Levasseur and Schally, {Andrew V}",
year = "1974",
month = "12",
day = "1",
language = "English",
volume = "52",
pages = "1012--1019",
journal = "Canadian Journal of Physiology and Pharmacology",
issn = "0008-4212",
publisher = "National Research Council of Canada",
number = "5",

}

TY - JOUR

T1 - Characteristics of the plasma disappearance of [3H] thyrotropin releasing hormone in the rat

AU - Dupont, A.

AU - Labrie, F.

AU - Levasseur, L.

AU - Schally, Andrew V

PY - 1974/12/1

Y1 - 1974/12/1

N2 - Thirty seconds after an intravenous injection of 0.5 ml of 2.5 x 10-6M [3H]thyrotropin releasing hormone (TRH) to male rats, the radioactivity was distributed in an apparent volume of 134 ml, thus reflecting its rapid disappearance from the blood. The pattern of plasma radioactivity after intravenous injection of [3H]TRH could be represented by the sum of 3 exponentials, suggesting a 3 compartment model of plasma disappearance of radioactivity. The half life of TRH and of its metabolites calculated from the first exponential curve was 2.2 min. Thin layer electrophoresis of methanol extracts of plasma collected 1, 7, 20, and 30 min after injection of [3H]TRH showed that the neurohormone was transformed into TRH free acid in proportions of 58, 58, 52 and 47%, respectively. The first 60 min seemed to be the most important period for elimination of the hormone and its metabolites by the kidney. In fact, 60 min after injection, 38% of the total radioactivity was found in the bladder. At 2, 15 and 30 min after injection of [3H]TRH, high accumulation of radioactivity occurred in kidneys, pineal gland, and anterior and posterior (including intermediate) lobes of the pituitary. High levels of radioactivity were seen in kidneys and anterior pituitary gland at all time intervals studied.

AB - Thirty seconds after an intravenous injection of 0.5 ml of 2.5 x 10-6M [3H]thyrotropin releasing hormone (TRH) to male rats, the radioactivity was distributed in an apparent volume of 134 ml, thus reflecting its rapid disappearance from the blood. The pattern of plasma radioactivity after intravenous injection of [3H]TRH could be represented by the sum of 3 exponentials, suggesting a 3 compartment model of plasma disappearance of radioactivity. The half life of TRH and of its metabolites calculated from the first exponential curve was 2.2 min. Thin layer electrophoresis of methanol extracts of plasma collected 1, 7, 20, and 30 min after injection of [3H]TRH showed that the neurohormone was transformed into TRH free acid in proportions of 58, 58, 52 and 47%, respectively. The first 60 min seemed to be the most important period for elimination of the hormone and its metabolites by the kidney. In fact, 60 min after injection, 38% of the total radioactivity was found in the bladder. At 2, 15 and 30 min after injection of [3H]TRH, high accumulation of radioactivity occurred in kidneys, pineal gland, and anterior and posterior (including intermediate) lobes of the pituitary. High levels of radioactivity were seen in kidneys and anterior pituitary gland at all time intervals studied.

UR - http://www.scopus.com/inward/record.url?scp=0016259861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016259861&partnerID=8YFLogxK

M3 - Article

C2 - 4214597

AN - SCOPUS:0016259861

VL - 52

SP - 1012

EP - 1019

JO - Canadian Journal of Physiology and Pharmacology

JF - Canadian Journal of Physiology and Pharmacology

SN - 0008-4212

IS - 5

ER -