Characteristics of rat chloroma in culture

Adel A Yunis, G. K. Arimura, Judith Ratzan

Research output: Contribution to journalArticle

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Abstract

Rat chloroma cells were propagated in permanent suspension cultures and grown in Dulbecco's modified Eagle's medium with 10% fetal calf serum and 2.5% horse serum. Several lines were established; the one of longest duration, Mia C51, has been maintained for over 18 months and has undergone over 100 transfers. Mia C51 cells have a doubling time of 12 hr and maintain many of the properties of the parent tumor, including the characteristic greenish color with high myeloperoxidase activity, an aneuploid chromosomal pattern, and intact tumorigenicity. They will uniformly produce greenish chloroma tumors when injected into newborn rats. Electron microscopic examination of chloroma tumors and the cultured cells derived from them reveal the presence of extracellular mature and immature type C virus particles morphologically typical of oncornaviruses. Chloroma cells obtained from tumors that lost their alkaline phosphatase activity after repeated transfer regain full activity in culture. Studies using the antigen antibody crossed electrophoresis indicate that the loss of alkaline phosphatase activity represents a true decrease in alkaline phosphatase protein, which is restored under culture conditions. The availability of a permanent chloroma cell line in culture that maintains the biological properties of the parent tumor provides a useful model for the study of myeloid leukemia.

Original languageEnglish (US)
Pages (from-to)337-345
Number of pages9
JournalCancer Research
Volume35
Issue number2
StatePublished - 1975

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Myeloid Sarcoma
Alkaline Phosphatase
Neoplasms
Cultured Tumor Cells
Eagles
Myeloid Leukemia
Aneuploidy
Retroviridae
Serum
Virion
Peroxidase
Horses
Electrophoresis
Suspensions
Color
Electrons
Antigens
Cell Line
Antibodies
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yunis, A. A., Arimura, G. K., & Ratzan, J. (1975). Characteristics of rat chloroma in culture. Cancer Research, 35(2), 337-345.

Characteristics of rat chloroma in culture. / Yunis, Adel A; Arimura, G. K.; Ratzan, Judith.

In: Cancer Research, Vol. 35, No. 2, 1975, p. 337-345.

Research output: Contribution to journalArticle

Yunis, AA, Arimura, GK & Ratzan, J 1975, 'Characteristics of rat chloroma in culture', Cancer Research, vol. 35, no. 2, pp. 337-345.
Yunis AA, Arimura GK, Ratzan J. Characteristics of rat chloroma in culture. Cancer Research. 1975;35(2):337-345.
Yunis, Adel A ; Arimura, G. K. ; Ratzan, Judith. / Characteristics of rat chloroma in culture. In: Cancer Research. 1975 ; Vol. 35, No. 2. pp. 337-345.
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