Characteristics of Perforin Expressing Lymphocytes Within the First Trimester Decidua of Human Pregnancy

DANIEL RUKAVINA, GORDANA RUBEŠA, LEA GUDELJ, HERMAN HALLER, ECKHARD R. PODACK

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

PROBLEM: The number of perforin (P)-positive cells in decidua of pregnancy is larger than that observed in any other pathological condition. The aim was to investigate the distribution and the phenotype of P+ cells. METHOD: Decidual tissue was obtained from the first trimester vaginal termination of pregnancy. Tissue distribution of P+ cells was analyzed by immunohistochemistry. The method for simultaneous measurement of P and cell surface is presented. RESULTS: There is no difference in number and distribution of P+ cells between decidua basalis (DB) and decidua parietalis (DP). The percentage of P+ decidual lymphocytes (DL) is two times higher than in peripheral blood lymphocytes (PBL) (55% vs. 27%), and the prevalent phenotype is CD3- CD4- CD8- CD2+ (95%) CD11c+ (68%) and CD56+ (82%). CD56(bright+) DL are also P(bright+) this is the largest DL subpopulation (42.4% DL). Two different subpopulations of CD8+ DL exist: 1) CD8(bright+), which are CD3+ CD56- P- and 2) CD8(dim+), which are CD3- CD56+ P+. CONCLUSION: P expressing DL are prevalently nonclassical NK cells (CD16-) with low cytolytic activity but fully equipped with potent cytolytic machinery (P(bright+)). There are no classical cytotoxic lymphocytes (CTL) (CD3+ CD8+ P+) in the decidua, and all CD8+ P+ cells are CD3- CD56+. The number of P+ cells is even higher in DP in the vicinity of noninvasvie trophoblast, than in DB.

Original languageEnglish (US)
Pages (from-to)394-404
Number of pages11
JournalAmerican Journal of Reproductive Immunology
Volume33
Issue number5
DOIs
StatePublished - May 1995

Keywords

  • CTL
  • Decidua
  • NK
  • Perforin
  • Perforin detection by FACS

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

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