Characteristics of fibrinolysin secreted by cultured rat breast carcinoma cells

M. C. Wu, D. R. Schultz, G. K. Arimura, M. A. Gross, Adel A Yunis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fibrinolysin secreted by cultured R2426 rat breast carcinoma cells has been purified and partially characterized. Three methods of assay were used: the hydrolysis of [125I]fibrinogen and N-p-tosyl-l-arginine methyl ester (TAME), and clearing of fibrin-agar plates (heated and unheated). Two types of fibrinolytic activity were identified: a plasminogen activator and a direct fibrinolysin. The fibrinolysin had direct hydrolytic action on plasminogen-free fibrinogen, fibrin, and TAME. The products of fibrinogen hydrolysis by the tumor cell fibrinolysin differed from those of human plasmin. It was heat stable (60°C, 12 h), and was inhibited by ε-aminocaproic acid and soybean trypsin inhibitor. Ethylenediaminetetraacetate (EDTA) inhibited the action of the tumor cell enzyme on TAME, but not on human fibrinogen. The direct fibrinolytic activity could be separated from plasminogen activator activity by gel filtration on Sephadex G-200, and the molecular weight was estimated to be approx. 110 000 D.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalExperimental Cell Research
Volume96
Issue number1
DOIs
StatePublished - Jan 1 1975

Fingerprint

Fibrinolysin
Fibrinogen
Breast Neoplasms
Plasminogen Activators
Fibrin
Hydrolysis
Aminocaproic Acid
Trypsin Inhibitors
Plasminogen
Soybeans
Agar
Gel Chromatography
Neoplasms
Hot Temperature
Molecular Weight
Enzymes
arginine methyl ester

ASJC Scopus subject areas

  • Cell Biology

Cite this

Characteristics of fibrinolysin secreted by cultured rat breast carcinoma cells. / Wu, M. C.; Schultz, D. R.; Arimura, G. K.; Gross, M. A.; Yunis, Adel A.

In: Experimental Cell Research, Vol. 96, No. 1, 01.01.1975, p. 37-46.

Research output: Contribution to journalArticle

Wu, M. C. ; Schultz, D. R. ; Arimura, G. K. ; Gross, M. A. ; Yunis, Adel A. / Characteristics of fibrinolysin secreted by cultured rat breast carcinoma cells. In: Experimental Cell Research. 1975 ; Vol. 96, No. 1. pp. 37-46.
@article{cc14a95bf07449b99464fedc5c899fdb,
title = "Characteristics of fibrinolysin secreted by cultured rat breast carcinoma cells",
abstract = "Fibrinolysin secreted by cultured R2426 rat breast carcinoma cells has been purified and partially characterized. Three methods of assay were used: the hydrolysis of [125I]fibrinogen and N-p-tosyl-l-arginine methyl ester (TAME), and clearing of fibrin-agar plates (heated and unheated). Two types of fibrinolytic activity were identified: a plasminogen activator and a direct fibrinolysin. The fibrinolysin had direct hydrolytic action on plasminogen-free fibrinogen, fibrin, and TAME. The products of fibrinogen hydrolysis by the tumor cell fibrinolysin differed from those of human plasmin. It was heat stable (60°C, 12 h), and was inhibited by ε-aminocaproic acid and soybean trypsin inhibitor. Ethylenediaminetetraacetate (EDTA) inhibited the action of the tumor cell enzyme on TAME, but not on human fibrinogen. The direct fibrinolytic activity could be separated from plasminogen activator activity by gel filtration on Sephadex G-200, and the molecular weight was estimated to be approx. 110 000 D.",
author = "Wu, {M. C.} and Schultz, {D. R.} and Arimura, {G. K.} and Gross, {M. A.} and Yunis, {Adel A}",
year = "1975",
month = "1",
day = "1",
doi = "10.1016/S0014-4827(75)80034-6",
language = "English",
volume = "96",
pages = "37--46",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Characteristics of fibrinolysin secreted by cultured rat breast carcinoma cells

AU - Wu, M. C.

AU - Schultz, D. R.

AU - Arimura, G. K.

AU - Gross, M. A.

AU - Yunis, Adel A

PY - 1975/1/1

Y1 - 1975/1/1

N2 - Fibrinolysin secreted by cultured R2426 rat breast carcinoma cells has been purified and partially characterized. Three methods of assay were used: the hydrolysis of [125I]fibrinogen and N-p-tosyl-l-arginine methyl ester (TAME), and clearing of fibrin-agar plates (heated and unheated). Two types of fibrinolytic activity were identified: a plasminogen activator and a direct fibrinolysin. The fibrinolysin had direct hydrolytic action on plasminogen-free fibrinogen, fibrin, and TAME. The products of fibrinogen hydrolysis by the tumor cell fibrinolysin differed from those of human plasmin. It was heat stable (60°C, 12 h), and was inhibited by ε-aminocaproic acid and soybean trypsin inhibitor. Ethylenediaminetetraacetate (EDTA) inhibited the action of the tumor cell enzyme on TAME, but not on human fibrinogen. The direct fibrinolytic activity could be separated from plasminogen activator activity by gel filtration on Sephadex G-200, and the molecular weight was estimated to be approx. 110 000 D.

AB - Fibrinolysin secreted by cultured R2426 rat breast carcinoma cells has been purified and partially characterized. Three methods of assay were used: the hydrolysis of [125I]fibrinogen and N-p-tosyl-l-arginine methyl ester (TAME), and clearing of fibrin-agar plates (heated and unheated). Two types of fibrinolytic activity were identified: a plasminogen activator and a direct fibrinolysin. The fibrinolysin had direct hydrolytic action on plasminogen-free fibrinogen, fibrin, and TAME. The products of fibrinogen hydrolysis by the tumor cell fibrinolysin differed from those of human plasmin. It was heat stable (60°C, 12 h), and was inhibited by ε-aminocaproic acid and soybean trypsin inhibitor. Ethylenediaminetetraacetate (EDTA) inhibited the action of the tumor cell enzyme on TAME, but not on human fibrinogen. The direct fibrinolytic activity could be separated from plasminogen activator activity by gel filtration on Sephadex G-200, and the molecular weight was estimated to be approx. 110 000 D.

UR - http://www.scopus.com/inward/record.url?scp=0016598166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016598166&partnerID=8YFLogxK

U2 - 10.1016/S0014-4827(75)80034-6

DO - 10.1016/S0014-4827(75)80034-6

M3 - Article

VL - 96

SP - 37

EP - 46

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -