Characteristics of complement receptor-bearing cells in the spleens of tumor-bearing mice

R. S. Epstein, Diana M Lopez, M. M. Sigel, G. D. Ross

Research output: Contribution to journalArticle

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Abstract

In the course of mammary tumor development, a population of nylon nonadherent cells with CR appears in the spleens of tumor-bearing mice although none are ever detected in normal mice. These cells apparently arise in response to immunologic stimulation. In a series of studies we have further characterized subsets of T cells (CR+ and CR-) with regard to their responses to mitogens in the lymphocyte transformation assay. Nylon column nonadherent cells from the spleens of tumor-bearing mice were rosetted in a complement receptor assay using EAC rosetting, and CR+ cells were separated from CR- by centrifugation in a discontinuous Ficoll gradient. CR+ T cells responded strongly to PHA and Con A and in addition responded to LPS, an activity not usually associated with conventional T cells. In contrast, CR- T cells from tumor-burdened mice responded to PHA but failed to respond to Con A or LPS.

Original languageEnglish
Pages (from-to)1524-1527
Number of pages4
JournalJournal of Immunology
Volume121
Issue number4
StatePublished - Dec 1 1978

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Complement Receptors
Spleen
Nylons
T-Lymphocytes
Neoplasms
Ficoll
T-Lymphocyte Subsets
Lymphocyte Activation
Centrifugation
Mitogens
Immunization
Breast Neoplasms
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Characteristics of complement receptor-bearing cells in the spleens of tumor-bearing mice. / Epstein, R. S.; Lopez, Diana M; Sigel, M. M.; Ross, G. D.

In: Journal of Immunology, Vol. 121, No. 4, 01.12.1978, p. 1524-1527.

Research output: Contribution to journalArticle

Epstein, R. S. ; Lopez, Diana M ; Sigel, M. M. ; Ross, G. D. / Characteristics of complement receptor-bearing cells in the spleens of tumor-bearing mice. In: Journal of Immunology. 1978 ; Vol. 121, No. 4. pp. 1524-1527.
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