Characteristics of apoptotic cell death induced by coxsackievirus B in permissive vero cells

Jeonghyun Ahn, Chul Hyun Joo, Ilsun Seo, Dong Hou Kim, Hea Nam Hong, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Coxsackievirus B (CVB) causes a wide spectrum of human diseases which are closely associated with direct destruction of infected cells. We investigated the morphological and biochemical characteristics of CPEs in permissive Vero cells caused by different CVB serotypes. Regardless of serotype, the infected cells experienced similar degrees of CPEs within 24 h postinfection (p.i.). Using both Hoechst 33342 staining and transmission electron microscopy, we consistently observed morphological properties of apoptosis, heavily condensed nuclei and subsequent chromatin condensation into the periphery of the nuclei within 12 h p.i. Moreover, we noticed typical oligonucleosomal DNA fragmentation, while productive CVB multiplication was accomplished within 6 h p.i. prior to an apoptotic signal. Caspase inhibitor significantly prohibited nuclear changes due to apoptosis with no influence on virus production and cell death, demonstrating that all the CVBs induced more than one type of pathological effect, including apoptotic alteration in permissive Vero cells.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalIntervirology
Volume46
Issue number4
DOIs
StatePublished - Sep 5 2003
Externally publishedYes

Fingerprint

Human Enterovirus B
Vero Cells
Cell Death
Apoptosis
Caspase Inhibitors
DNA Fragmentation
Transmission Electron Microscopy
Chromatin
Staining and Labeling
Viruses
Serogroup

Keywords

  • Apoptosis
  • Coxsackievirus B
  • Cytopathology
  • Vero cell
  • Virus production

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Ahn, J., Joo, C. H., Seo, I., Kim, D. H., Hong, H. N., Kim, Y. K., & Lee, H. (2003). Characteristics of apoptotic cell death induced by coxsackievirus B in permissive vero cells. Intervirology, 46(4), 245-251. https://doi.org/10.1159/000072435

Characteristics of apoptotic cell death induced by coxsackievirus B in permissive vero cells. / Ahn, Jeonghyun; Joo, Chul Hyun; Seo, Ilsun; Kim, Dong Hou; Hong, Hea Nam; Kim, Yoo Kyum; Lee, Heuiran.

In: Intervirology, Vol. 46, No. 4, 05.09.2003, p. 245-251.

Research output: Contribution to journalArticle

Ahn, J, Joo, CH, Seo, I, Kim, DH, Hong, HN, Kim, YK & Lee, H 2003, 'Characteristics of apoptotic cell death induced by coxsackievirus B in permissive vero cells', Intervirology, vol. 46, no. 4, pp. 245-251. https://doi.org/10.1159/000072435
Ahn, Jeonghyun ; Joo, Chul Hyun ; Seo, Ilsun ; Kim, Dong Hou ; Hong, Hea Nam ; Kim, Yoo Kyum ; Lee, Heuiran. / Characteristics of apoptotic cell death induced by coxsackievirus B in permissive vero cells. In: Intervirology. 2003 ; Vol. 46, No. 4. pp. 245-251.
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