Abstract
In a patient with hypogonadotropic hypogonadism treated with luteinizing hormone releasing hormone (LHRH), secondary failure of both subjective and hormone responses occurred at the time of appearance of binding of 125I LHRH by the patient's serum. On electrophoresis of the patient's serum with 125I LHRH, label was found only in the gamma globulin region. 125I LHRH added to the patient's serum was precipitated by sheep antihuman immunoglobulin G (anti IgG) but not by sheep anti human immunoglobulin M (anti IgM). Competitive displacement of 125I LHRH by unlabeled LHRH was demonstrated while TSH releasing hormone (TRH), somatostatin and rat pituitary hormones showed no displacement when tested at concentrations 5 x 106 greater than that of LHRH. Studies using 14 different analogs of LHRH revealed that those with changes at the carboxy terminus showed binding similar to LHRH. It is concluded that IgG antibody to LHRH was produced in this patient by repeated administration of synthetic LHRH. It is further concluded that antibody specificity is directed toward the N terminus region.
| Original language | English |
|---|---|
| Pages (from-to) | 784-790 |
| Number of pages | 7 |
| Journal | Journal of Clinical Endocrinology and Metabolism |
| Volume | 44 |
| Issue number | 4 |
| State | Published - Dec 1 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology, Diabetes and Metabolism
Cite this
Characteristics of antibody produced during chronic treatment with LHRH. / Brown, G. M.; van Loon, G. R.; Hummel, B. C W; Grota, L. J.; Arimura, A.; Schally, Andrew V.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 44, No. 4, 01.12.1977, p. 784-790.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characteristics of antibody produced during chronic treatment with LHRH
AU - Brown, G. M.
AU - van Loon, G. R.
AU - Hummel, B. C W
AU - Grota, L. J.
AU - Arimura, A.
AU - Schally, Andrew V
PY - 1977/12/1
Y1 - 1977/12/1
N2 - In a patient with hypogonadotropic hypogonadism treated with luteinizing hormone releasing hormone (LHRH), secondary failure of both subjective and hormone responses occurred at the time of appearance of binding of 125I LHRH by the patient's serum. On electrophoresis of the patient's serum with 125I LHRH, label was found only in the gamma globulin region. 125I LHRH added to the patient's serum was precipitated by sheep antihuman immunoglobulin G (anti IgG) but not by sheep anti human immunoglobulin M (anti IgM). Competitive displacement of 125I LHRH by unlabeled LHRH was demonstrated while TSH releasing hormone (TRH), somatostatin and rat pituitary hormones showed no displacement when tested at concentrations 5 x 106 greater than that of LHRH. Studies using 14 different analogs of LHRH revealed that those with changes at the carboxy terminus showed binding similar to LHRH. It is concluded that IgG antibody to LHRH was produced in this patient by repeated administration of synthetic LHRH. It is further concluded that antibody specificity is directed toward the N terminus region.
AB - In a patient with hypogonadotropic hypogonadism treated with luteinizing hormone releasing hormone (LHRH), secondary failure of both subjective and hormone responses occurred at the time of appearance of binding of 125I LHRH by the patient's serum. On electrophoresis of the patient's serum with 125I LHRH, label was found only in the gamma globulin region. 125I LHRH added to the patient's serum was precipitated by sheep antihuman immunoglobulin G (anti IgG) but not by sheep anti human immunoglobulin M (anti IgM). Competitive displacement of 125I LHRH by unlabeled LHRH was demonstrated while TSH releasing hormone (TRH), somatostatin and rat pituitary hormones showed no displacement when tested at concentrations 5 x 106 greater than that of LHRH. Studies using 14 different analogs of LHRH revealed that those with changes at the carboxy terminus showed binding similar to LHRH. It is concluded that IgG antibody to LHRH was produced in this patient by repeated administration of synthetic LHRH. It is further concluded that antibody specificity is directed toward the N terminus region.
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M3 - Article
C2 - 321467
AN - SCOPUS:0017618398
VL - 44
SP - 784
EP - 790
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 4
ER -